G. Frederick, Castellana Barbara

F. Departament-de, Departament de Biologia Cellular, Fisiologia i d'Immunologia, Bellaterra, issue.4

S. Tatiana, Wuertz Sven (2), Bayunova Liubov (1)

, Central laboratory of fish reproduction, vol.24, 197022.

, Leibniz-Institute for Freshwater Ecology and Inland Fisheries, Müggelseedamm, vol.310, p.12587

G. Berlin, Russia BACKGROUND: In fish, maturation inducing steroids (MISs) regulate the resumption of oocyte meiosis and induce germinal vesicle break down (GVBD). Presumably, MIS belongs to the C21 steroids, comprising gestagens and glucocorticoids. In sturgeon, several C21 steroids are produced by gonads during final maturation and are able to induce GVBD in vitro, 199034.

, The effect of different concentrations of progesterone (P4), 11-deoxycortisole (S) and 11-deoxycortisone (P21) on GVBD were quantified in an in vitro assay. Triplicates of 10 ovarian follicles, each in 2.5 ml media were exposed to 600, 800, 1000 or 1200 nM of P4, S and P21 for 5 min, followed by 24 h incubation in steroid-free media. In a second experiment, 10 follicles (in duplicate) were exposed for 1 min. After incubation, follicles were fixed in formalin-ethanol solution and scored for GVBD. Controls were incubated in steroid free media. RESULTS: After short-time exposure (1 and 5 min), all C21 steroids tested were able to induce GVBD in a dose-dependent manner in sterlet follicles. After 5 min of exposure, P4 was more effective in inducing GVBD than S and P21, METHODS: Ovarian follicles were taken from three gravid sterlet females (Acipenser rhutenus L.) and incubated in Leibovitz's L-15 media (Life Technologies; pH adjusted to 7.8) with penicillin G (0.03 g 1 -1 ) and streptomycin sulfate (0.05 g 1 -1 )

, CONCLUSION: The results clearly demonstrate that P4 is more effective in the stimulation of GVBD than S and P21. These findings are congruent with previous studies in other sturgeon species, where P4 induced GVBD at lower concentrations than S, vol.17

, Domestication and GnRHa induced-spawning of meagre

D. Neil, ;. , and C. Ignacio, Aguilera Cristóbal (1), Norambuena Fernando (1)

S. Irta and . Carles-de-la-rapita, Aimal Biology and Fish Disease Diagnostic Service, Facultat de Veterinaria, Tarragona, vol.43540, issue.2, p.186

S. Adachi and . P61, , vol.72, p.160

S. Aegerter, C. Aguilera, and M. Agulleiro, , p.219

S. Aït-aïssa, , vol.14, p.166

J. Aizen, , p.21

M. Akiyoshi and S. Alavi, , p.121

D. Alsop, O57 Alvarenga E: P120 Amano H: O58, vol.180, p.182

A. Amerand, P24 Andersson E: O37, P31, P51, P113, P114, P116, vol.117, p.119

M. André, , p.146

, Anglade I: O9, vol.15, p.166

J. Y. Aoki, , p.171

, Aprea A: P129 Arana NC: P161 Araujo RV: P138 Aroua S: P27, p.28

A. Arukwe, , vol.47, p.175

M. Ashida, P72 Askarian F: P213 Assis L: P100 Asturiano JF: P204, P205, p.209

R. K. Atta, P161 Avella M: P203 Avitan A: O10 Babiak I: O44, p.71

, Babiak J: O44, p.71

P. Babin, , p.146

A. A. Badiane, P220 Baek HJ: P170 Ballevona A: P145 Baloche S: P12, p.24

M. Barney and J. F. Baroiller, , vol.21, p.152

M. J. Bayarri, P7 Bayunova L: P187, p.207

B. R. Beckman, O67 Beintema J: P201 Belmonte A: O41, p.62

S. Benedet, , p.116

T. J. Benfey, O40 Berejikian BA: O67 Berishvili G: O12, p.152

S. Bernard-samain, L. Besseau, M. Betancourt-lozano, and R. K. Bhandari, P125 Bibenko A: P208 Bibenko O: P208 Bienvenu D: O21 Biran J: O10 Björnsson BT: P31, vol.36, p.116

M. J. Blanchard, O40 Blasco M: P42 Blázquez M: P76 Bloch C: O10 Bobe J: O48, O50, P52, P123, P130, P133, vol.157, p.183

G. Boeuf, A. Böhne, and C. Bon-chu, P64 Bonnet E: O48, P147, P157 Booth A: P150 Breton B: O35, P195 Bridges CR: O41, vol.62, p.129

, Brion F: O9, vol.14, p.166

S. Brown, O57 Brunet F: O16 Bryan M: P174 Burton M: P197 Cabas I: O30 Cabrita E: P101, p.210

L. Cahill and R. M. Calvo, P39 Campbell B: O67 Campos-Ramos R: P212 Campos-Silva S: P100 Canarin M: P215 Cánepa M: P29 Caraty A: P8 Carazo I: P216, p.219

R. Carbo and A. Carcagno, P29 Cardenas R: P151 Cardillo E: O3, vol.2, p.88

O. Carnevali, , vol.124, p.203

M. Carrillo, . P1, and . P126, , vol.142, p.179

T. Carroll, C. Carter, and M. Carvalho, P137 Casselman J: P145 Castellana B: P183 Cathrine K: O28 Cauty C: O24 Cavileer T: P153 Cerdà J: O49, P70, P146, vol.217, p.219

J. M. Cerdá-reverter, P179 Cerqueira V: P215 Chan KM: O13 Chang CF: O26, P55, vol.156, p.185

, Chatziandreou N: O55 Chaves-Pozo E: O30 Chavez M: P151 Chemineau P: O4 Chenais N: P133, p.158

G. Cooper, P89 Corriero A: O41, vol.62, p.129

A. Cossins, , vol.55, p.17

J. Cosson, , p.102

A. Couloux, O16 Crespel A: P130 Cross N: O40 Cui J: P125 Cui M: P19 Cuppen E: P91 D'Cotta H: O12, vol.21, p.152

R. Da-cuña, K. Dabrowski, and B. Davail-cuisset, , p.86

D. Davidson, P201 Davie A: O5, vol.1, p.211

L. Davis and C. Conto, , vol.148, p.149

G. De-metrio, , vol.62, p.129

G. Degani, O63 Denier X: P173 Depincé A: P133, p.158

R. Dominguez, , p.198

R. Dosch and M. A. Driancourt, P195 Du JL: O26 Dufour S: O4, P12, P24, P27, vol.28, p.156

R. Dugué, , p.161

J. Duitman and N. Duncan, , vol.216, p.219

F. Duponchelle, , p.161

M. El, A. Elizur, and T. Endo, , p.160

S. Engrola and W. J. Enright, P195 Epler P: P22, p.115

E. Eppler, , vol.12, p.152

D. Esquerré, A. Estevez, and J. M. Exbrayat, , p.149

M. Fabra, O49 Falcón J: P3, P5, P38, P39 Fang YX: O55 Fauvel C: O41, O62, P93, vol.106, p.130

H. Feitsma, P91 Felip A: P179 Fernández-Alacid L: O30 Fernandino JI: P57, vol.58, p.75

R. N. Finn, O61 Fitzgibbon Q: O69 Fleidervish I: O10 Fontaine P: O70, vol.107, p.108

J. Forgue, P139 Fostier A: O50, O70, P65, vol.73, p.157

E. Fraboulet, P130 França L: O42, P91, P92, vol.100, p.120

M. Frantzen, P167 Froschauer A: O16 Fuentes J: O2 Fujimoto Y: P199 Fujioka T: P77 Fujita T: O58, p.180

B. Funkenstein, P41 Galiana-Arnoux D: O16 Galima M: O8 Garcia A: O41, O62 García-Alcaraz A: O30 García-Ayala A: O30 Garcia-Gasca A: P159, p.163

J. N. Gardeur, , vol.70, p.107

F. Garren and A. Gautier-stein, , p.60

W. Ge, O45 Gela D: O73, P67, vol.97, p.102

K. Gen, V. Ghole, and M. R. Ghomi, P206 Gioacchini G: P203 Goetz FW: O46, vol.48, p.183

M. Golan, O23 Gómez A: O36, P126, vol.127, p.142

P. Gonthier and J. L. Gonzalez, P151 Gordin H: O41, p.62

Y. Gothilf, M. Gouda, and R. J. Gould, P77 Goupil AS: P8, vol.118, p.123

R. Grabic, E. Grau, and A. L. Groison, , p.96

D. Grzegorz and G. Guan, , p.56

H. Guerrero and . O3, , vol.2, p.88

. Guerrero-tortolero and . Da, P212 Guiguen Y: O24, O50, P52, P63, P65, P66, vol.73, p.123

L. Guilgur, , p.75

M. Guillevic, , p.73

A. Gutiérrez, O18 Gutiérrez J: P163 Gutnick M: O10 Gutzeit H: O21, p.49

J. M. Guzmán, , vol.38, p.162

H. R. Habibi, P168 Haffray P: O32, p.195

J. Hamackova, K. Hamre, and R. Hanna, , p.184

C. Harris and H. Hashimoto, , p.186

R. S. Hattori, , vol.58, p.77

T. M. Haug, , p.26

T. Haugen and Y. Hayakawa, , p.194

G. Heinnisch and E. Henrotte, , p.190

A. C. Henshaw, P79 Hernandez R: P159 Hernández-Cornejo R: P163 Herráez MP: P143 Herrera P: O6, p.5

K. Higuchi and M. Higuchi, , p.82

T. Higuchi, , p.155

E. M. Hill, P173 Hinfray N: O14, p.166

T. Hirai, , vol.51, p.68

T. Hirano, P164 Hodne K: P26 Holt GJ: P203 Holtz W: P141 Hori H: P48 Horiguchi R: P46 Horiuchi Y: P87, p.186

S. M. Hossain, P60 Houlgatte R: O29, p.65

C. Hu and H. Hu, P40 Hu W: P20 Huang B: P59 Huang J: P30, p.122

X. Huang and Y. S. Huang, P27 Hubermont P: P105 Huertas M: P70 Hulak M: O73, vol.67, p.103

A. Hurvitz, O63 Hwang IJ: P170 Hwei-jan H: P64 Ibarra-Castro L: P159 Ideuchi H: P180 Ijiri S: O19, vol.61, p.160

, Iliev D: O46, P183 IMV T: O32 Inbaraj M: P14, p.18

M. Inoue, P180 Ishizawa S: P33 Isorna E: P38, p.39

S. Jamin, K. Jan, and S. R. Jeng, , p.185

B. Jiao and L. F. Jimenez, P151 Jitka H: P192 Johnston T: P145 Jolivet A: P96 Jordal AEO: P36 Jordal S: P51 Kobayashi T: O19, vol.80, p.90

M. Kocour, K. Kohlmann, and J. Kolarevic, O61 Kollar S: P145 Komrakova M,: P141 Konno S: P191 Koop B: P89 Kortner Trond M, p.47

L. Labbé, , p.181

S. Lacerda and . O42, , p.100

, Lacomme S: P86 Lacuisse M: P210 Lado W: P17 Ladstein S: P176 Landers D: O25 Lange A: O55, p.79

S. E. Lankford, P189 Lareyre JJ: O29, O35, P8, P15, P16, vol.52, p.123

D. Larsen and E. L. Lau, P125 Le Bail PY: O70, P8, vol.133, p.158

L. Belle and N. , , p.28

L. Callennec and C. , , p.147

L. Coz and J. R. , P96 Le Gac F: O29, O35, P8, P118, vol.123, p.135

M. Leal, , p.92

Y. D. Lee, , p.171

Y. H. Lee, O26 Lee YX: O26 Legendre M: P98, vol.99, p.161

. Leggett, P145 Legros C: O4 Lepage M: P86 Lethimonier C: P15, p.16

. Levavi-sivan-b:-o10, , p.150

L. Levi, , vol.52, p.53

W. S. Li, P19 Liarte-Lastra S: O30 Lim BS: P171 Lin D: P53 Lin H: P40, vol.122, p.154

L. Hr:-p19 and . Linhart-o:-o31, , p.136

A. Lister and J. Liu, , vol.66, p.63

X. Liu and Y. Liu, O66 Liu Z: P59 Lo NF: P23 Lokman PM: P90, p.160

J. López-olmeda, M. Losurdo, E. Lubzens, J. Luckenbach, K. Luo et al., , p.190

X. Ma and G. Maack, , p.54

S. Mackenzie and M. C. Maggese, , p.29

J. Maillard, G. Maisse, Y. Makiguchi, and J. Malcolm, P167 Male R: P51, p.176

B. Malpaux, O4 Maltret P: P173 Mañanós E: P162 Mancera JM: O2 Mandiki R: P105, p.107

F. Maradonna, , vol.124, p.203

F. Marco-jimenez, P209 Maria AN: P137 Marie M: P108 Marlatt V: P17 Marmignon MH: P16 Martinez I: O36 Martinez-Chavez C: P7 Martínez-Páramo S: P143 Martinez-Pastor F: P101 Martinez-Rodriguez G: O2 Martyniuk C: P17 Masaru N: P43 Matsubara H: P90 Matsubara T: O58, O59, P90, P165, vol.180, p.182

M. Matsuda, , vol.17, p.54

J. Matsumoto, O57 Matsumoto S: P132 Matsuoka M: P62 Maugars G: P85, p.200

C. Mazzora-de-quero, P211 Mechaly A: P9, p.104

, Meiri I: O62 Melard C: P69, P105, p.220

J. Menningen and J. Meseguer, O30 Migaud H: O5, P1, P7, vol.128, p.211

B. Migliarini and . Mikolajczyk, , p.195

M. Minghetti, , vol.5, p.7

K. Mizuno and A. Moen, P36 Mohamed J: O8 Molés G: P127, p.142

F. E. Montero, P216 Montfort J: O29 Morais S: P146 Moret O: O12 Morita T: P132 Morohashi K: O19 Mouriec K: O9, p.15

B. Mourot, J. Mraz, and A. Muhlia-melo, , p.212

V. Mulero and S. Muncaster, P119 Muñoz-Cueto J: O6, P3, P4, P5, vol.38, p.39

K. Murashita, P90 Muriach B: P179 Mylonas C: O41, O62, P106, vol.129, p.162

, Nadzialek S: P74 Nagae M: P155, vol.165, p.171

Y. Nagahama, , vol.125, p.178

K. Nagasawa, , vol.94, p.95

H. Nagaya, P132 Nagler JJ: P153 Nakamura M: O27, P46, vol.68, p.72

K. Nakao, P193 Nanda I: O16 Nash RDM: O72, p.202

L. Navarro-martín, , vol.18, p.76

R. M. Nazari, P206 Nebesarova J: P97 Neil SRE: O40 Nelson ER: P168 Nguyen T: O50 Nietrzeba M: O2 Nieveen M: O39 Nii H: P193 Nijenhuis W: P113 Nilsen TO: P36 Nishi A: P87, p.186

J. Nocillado and K. Nomura, P90 Norambuena F: P216 Norberg B: O28, O37, O38, P25, P71, P113, P114, P117, vol.119, p.162

B. Nyonge, O68 Nzau Matondo B: O65 Obregón MJ: P39 Ogawa S: P68 Ohkubo N: P165, p.182

H. Ohta, , p.131

T. Ohta, , p.84

K. Okubo, , vol.19, p.125

T. Okutsu and K. Okuzawa, P140 Olivotto I: P203 Opstad I: P119 Orban L: P60 Orfao LH: P137 Otero D: O49, p.217

S. Otomo, P194 Ouarour A : P4 Ovidio M: O65 Ovisi PM: P206 Ozaki Y: P82 Ozouf-Costaz C: O16 Pace M: O33 Padros F: P216 Pakdel F: O9, vol.14, p.15

, Palevitch O: O7 Páll M: P114 Palluel O: O14, p.166

, Palstra A: O39 Pandolfi M: P23 Pang Y: O33, p.19

N. Pankhurst, , vol.69, p.109

P. Pankhurst, N. Papandroulakis, and C. Park, P171 Park L: O67 Parsons JE: P153 Pasqualini C: P12 Patil J: P45 Patino R: P188 Paull G: O55, p.79

B. Paul-prasanth, , vol.17, p.54

, Penaranda DS: P204, vol.205, p.209

E. Pepey and . Perez, , vol.205, p.209

J. C. Pérez-cerezales-s:-p143-pérez-urbiola, P212 Pfennig F: O21, p.49

J. Philippart, Y. Piazza, F. Piferrer, . P9, and J. V. Planas, , vol.48, p.183

G. Poleo, , vol.88, p.214

, Policar T: P81, P107, P121, vol.136, p.192

P. Poncin, J. Popesku, and J. M. Porcher, , vol.14, p.166

J. Porta, P219 Porta JM: P219 Porteous L: P145 Pousao-Ferreira P, p.210

F. Powell and C. Pozzi-a:-p29-prignon, , p.220

, Psenicka M: P81, vol.97, p.121

G. Pyle, P145 Quillet E: P118 Radaelli G: P41 Ramos J: P162 Randuineau G: O24, p.52

P. Rault, P195 Rawson D: P139 Reading BJ: O59 Rebhan Y: P41 Regan C: P177 Reinecke M: O12, p.152

M. Reith, J. J. Rémy, and M. C. P135-rendón, , p.4

F. Resende, P100 Ricordel MJ : P123 Rigolino MG: P138 Rime H: P130 Ríos-Sicairos J: P163 Rocha A: O36, vol.126, p.127

M. Rodina, , p.136

G. Rosenlund and . Rouault, , p.218

, Rougeot C: P69, P105, p.220

J. Rutaisire and F. Saborido-rey, , p.198

F. J. Sánchez-vázquez, O6 Sand O: P26 Sandvik G: P25 Santamaria N: P129 Santos E: P47 Santosh W: P14, p.18

, Saori M: P43 Sato M: P72 Sauzet S: P5 Sawaguchi S: P182 Sbaihi M: P27 Schartl M: O16, p.49

, Schnabel D: O39 Schouten M: P201 Schreck C: O25 Schugardt C: O68 Schultheis C: O16 Schulz C: O68 Schulz R: O28, O37, P91, P92, P113, vol.117, p.128

, Sébert P: P24 Sebire M: P34 Segner H: P152 Ségurens B: O16 Selz Y: O16 Semenkova T: P187, p.207

A. Servili, , vol.6, p.38

K. I. Shiroyama, P165 Shirozu T: P48 Shmoish M: O52 Shved N: O12, P152 Simonich S: O25 Simpson C: O57 Sivan B: O53 Skaar K: P92 Skiftesvik AB: P119 Skjaeraasen JE: O72, p.202

, Skopal T: P41 Slotte A: P202 Soares F: P101 Socha M: P22, p.115

J. A. Sodahl, P175 Sohn YC: P132 Sohrabnejad M: P206 Sok-Keng T: P64 Sokolowska-Mikolajczyk M: P22, p.115

G. Somoza and . P42, , vol.58, p.75

H. Spaink and D. Spedicato, P129 Sreenivasan R: P60 Stefansson S: P36 Stoddard J: P153 Strobl-Mazzulla P: P57, p.75

C. A. Strüssmann, O22, P57, P58, vol.75, p.77

J. Subagja, , p.79

H. Ueda and . P32, , vol.33, p.193

, Unuma T: P90 Usami T: O27 Vagelli A: O71 Vallés R: P38 van Aerle R: P6, vol.10, p.47

C. Van-damme, P201 van den Thillart G: O39 Van Der Kraak G: O57, P177 van der Meeren, p.38

M. Vandeputte, O73 Vanegas G: P2 Vassallo R: O62 Vázquez R: P39 Vernier P: P12 Vettier A: P24 Vidal B : P12 Vignon X: P133 Vikingstad E: P114 Viñas J: O18, vol.9, p.104

, Vissio P: P23, P29 Viveiros ATM: P137, p.138

D. Vizziano, . O50, and . P42, , vol.123, p.172

S. Vlastimil, P192 Volff JN: O16, O21 von Schalburg K: P89 Vuillard JT: P107 Wang D: O19, P56, P59, vol.61, p.178

Z. Wang, P59 Wargelius A: P36 Watanabe S: P58, p.77

E. Watchorn, G. Weber, and C. Weil, P118 Weitkamp H: O68 Weltzien FA: O4, P12, P24, vol.25, p.26

M. Wenning, M. Wiegand, and S. Wiens, , p.37

E. Wit, F. Wu, and G. C. Wu, , vol.26, p.55

S. Wuertz and X. Xia, , p.37

H. Xiong, , p.37

H. Yambe, , vol.27, p.191

H. Yang and K. Yang, P19 Yang R: P47 Yano A: P89 Yaron Z: P21 Yen HF: P185 Yeo IK: P171 Yokoi KI: P131 Yokota M: P58, p.77

M. Yom-din-s:-o63-yoshikuni, O51 Yoshinaga N: P48 Yoshizaki G: O43, P89, P94, P95, vol.132, p.134

, Yueh WS: O26, vol.156, p.185

S. Zanuy and . P126, , vol.142, p.179

C. Zhang, D. Zhang, and L. Zhang, , p.53

T. Zhang and W. Zhang, , p.53

X. Zhang, P59 Zhang Y: P30, P53, P59, vol.122, p.154

H. Zhao, P54 Zhou L: O51, P56, vol.59, p.178

L. Y. Zhou, O19 Zhou Q: O16 Zhu P: P122 Zhu T: P53 Zhu Y: P169, p.184

Z. Y. Zhu, P20 Zhuo Q: P122 Zohar Y: O7, O41, vol.62, p.13