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Un modèle de variabilité fonctionnelle chez les arbres forestiers : le gène CCR d'eucalyptus

Abstract : Nucleotidic polymorphism of Cinnamoyl CoA Reductase (CCR) gene and its relation with lignin content is studied within a breeding population of Eucalyptus urophylla S.T. Blake (“Timor Mountain Gum”). The nearly full sequence (94%) are obtained for 15 parental trees. This gene (3220 bp) is highly polymorphic showing 131 single nucleotide polymorphism (SNP), 17 insertion-deletions (INDEL), 1 polyA sequence and a microsatellite site. Exons fragments encompass 10 non-synonymous SNPs, half of them within exon 5 (194 bp). Fifteen different haplotypes are reconstructed based on the polymorphism of exon 4 and intron 4. CCR promoting sequence (694 bp) including all the known regulatory sequences is described for the two alleles of one of the genitor trees displaying QTL and CCR gene colocalization in its genetic map. Five SNPs are present. Functional variability of the promoting sequence will be studied in planta through genetic modification of Arabidopsis thaliana. Lignin content was assessed within a sample of 35 full sib families (348 individuals) generated with the 15 parental trees, showing a high genetic additive control for this trait (h²=0.76). A new algorithm based on Reversible-jump MCMC was developed in order to implement association studies. Half of the progeny trees (208) were genotyped using the microsatellite fragment. The results show that a significant part of the observed genetic variance of lignin content is due to the nucleotide polymorphism of the studied gene. Those preliminary results look promising in order to develop early gene assisted selection for eucalyptus clones used as raw material in charcoal and paper production.
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  • HAL Id : hal-02821259, version 1
  • PRODINRA : 48800

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Jean-Marc Gion, Fréderic Mortier, Eric Mandrou, Paulo Ricardo Hein Gherardi, Tristan Costecalde, et al.. Un modèle de variabilité fonctionnelle chez les arbres forestiers : le gène CCR d'eucalyptus. 7. Colloque National: Ressources Génétiques, Oct 2008, Strasbourg, France. 17 p. ⟨hal-02821259⟩

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