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Bradford quantification of Glomalin-Related Soil Protein in coloured extracts of forest soils

Abstract : Glomalin-related soil protein (GRSP) is thought to represent a fraction of recalcitrant organic matter in soil. But it is recognized that the autoclaved-citrate extraction procedure causes the co-extraction of humic substances which interfere (directly and indirectly) with the Bradford colorimetric assay. The aim of this work was to propose a reliable quantification method of GRSP from forest soil, very rich in organic matter and therefore in colour. We estimated the quantities of GRSP in the topsoil (0–10 cm) of 102 French forests using five methods: i) direct calibration, reasoned dilution with colour correction, ii) direct calibration, reasoned dilution but no colour correction, iii) direct calibration, 1:2 dilution, no colour correction, iv) indirect calibration and v) dilution method. Our results concur that the interference caused by the co-extracted compounds is not related simply to either the colour of the extracts or total soil organic matter content. These findings suggest that for improved accuracy of GRSP estimates using the Bradford method, extracts should be diluted, and the pH-specific absorbance of coloured extracts should be subtracted.
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https://hal.inrae.fr/hal-02865912
Déposant : Dominique Fournier <>
Soumis le : vendredi 12 juin 2020 - 10:15:04
Dernière modification le : mercredi 14 octobre 2020 - 04:06:25

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G. Cissé, M. Essi, M. Nicolas, Siobhan Staunton. Bradford quantification of Glomalin-Related Soil Protein in coloured extracts of forest soils. Geoderma, Elsevier, 2020, 372, pp.114394. ⟨10.1016/j.geoderma.2020.114394⟩. ⟨hal-02865912⟩

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