Spatial heterogeneity in plant and soil properties plays a key role for biogeochemical cycling, nutrient losses and ecosystem function. Different management practices are expected to inducevaryinglevelsofspatialheterogeneityinagroecosystems,buttheeffectsofcontrastingbiomass removal regimes and herbivore species on grassland variability and spatial pattern have faced little attention. Wecarriedoutaspatially-explicitsamplingcampaignandgeostatisticalanalysestoquantify the spatial heterogeneity of the biomass and N in plants and soil for three management treatments (mowing, cattle grazing and sheep grazing) within a long-term grassland experiment. All plant and soil properties showed within-site variation, irrespective of management treatment. Within-site variationinplantvariablescouldberankedasgrazing>mowing. Cattlegrazingincreasedvariability in vegetation structure, soil mineral N and soil C:N compared with sheep grazing. In addition, the cattle-grazed field had a higher degree of spatial structure and a more coarse-grained pattern of spatial heterogeneity in plant properties than the sheep-grazed field. However, both grazing treatments showed spatial asynchrony in above- and below-ground responses to grazing. These results demonstrate the importance of herbivore species identity as a driver of grassland spatial heterogeneity, with implications for spatial uncoupling of nutrient cycles at the field scale.