, The coexistence of two different types of reference material will make the control laboratories' work harder and more expensive. The detection/quantitation methods for 281-24-236 x 3006-210-23 cotton and MON 88913 cotton were validated by the EURL-GMFF 13 using metric criteria rather than the speciesrelated specificity criteria required by ISO 21570 (ISO, 2008) and the ENGL guidance from the EURL-GMFF (ENGL, cotton and MON 88913 cotton is available from the JRC-IRMM 11 and AOCS 12 respectively, pp.281-305, 2008.

, HCB Scientific Committee points out that quantitation results ought to be calibrated according to the Praça-Fontes reference (Praça-Fontes et al., 2011) rather than the older Arumuganathan and Earle reference, 1991.

, The HCB Scientific Committee requests that these aspects relating to reference material, specificity and calibration of detection/quantitation methods for 281-24-236 x 3006-210-23 x MON 88913 cotton be reviewed in accordance with ISO standards and ENGL guidance

K. Arumuganathan, E. , and E. , Nuclear DNA content of some important plant species, Plant Molecular Biology Reporter, vol.9, pp.208-218, 1991.

. Engl, Definition of minimum performance requirements for analytical methods of GMO testing (European Network of GMO Laboratories, Community Reference Laboratory GM Food and Feed), p.8, 2008.

. Iso, Foodstuffs --Methods of analysis for the detection of genetically modified organisms and derived products --Quantitative nucleic acid based methods, Spanish Standard, vol.21570, 2008.

M. M. Praça-fontes, C. R. Carvalho, W. R. Clarindo, and C. D. Cruz, Revisiting the DNA C-values of the genome size-standards used in plant flow cytometry to choose the "best primary standards, Plant Cell Rep, vol.30, pp.1183-1191, 2011.

, See comments from ANSES forwarded to EFSA by the Ministry for the Economy, Finance and Foreign Trade

, from ANSES forwarded to EFSA by the Ministry for the Economy, Finance and Foreign Trade

, Institute for Reference Materials and Measurements: one of the seven institutes of the European Commission's, Joint Research Centre

, American Oil Chemists' Society: an association that promotes the sharing of scientific information and knowledge

, The European Commission Joint Research Centre's Community Reference Laboratory for GM Food and Feed was established by Regulation (EC) No 1829/2003 and is now called the EURL-GMFF (European Union Reference Laboratory for GM