Skip to Main content Skip to Navigation
Journal articles

New 16S rRNA primers to uncover Bdellovibrio and like organisms diversity and abundance

Abstract : Appropriate use and specific primers are important in assessing the diversity and abundance of microbial groups of interest. Bdellovibrio and like organisms (BALOs), that refer to obligate Gram-negative bacterial predators of other Gram-negative bacteria, evolved in terms of taxonomy and classification over the past two decades. Hence, some former primers have become inadequate while others are yet to be designed, for both PCR (especially with the advent of NGS) and qPCR approaches. Thus, to study BALOs' abundance and diversity in a variety of aquatic ecosystems, we designed in silico specific primer sets for each BALO genera and tested them in vitro on a variety of cultures and environmental samples. Also, we performed Sanger and Nano Miseq sequencing to reveal the exact degree of specificity of the most promising primers set. Here we report our success in designing specific primers for some BALOs genera, i.e. Bdellovibrio (PCR), Bacteriovorax (qPCR), Peredibacter (PCR).
Document type :
Journal articles
Complete list of metadata

Cited literature [47 references]  Display  Hide  Download
Contributor : Stéphan Jacquet <>
Submitted on : Thursday, September 10, 2020 - 11:28:09 AM
Last modification on : Saturday, April 3, 2021 - 3:28:00 AM


Files produced by the author(s)




Jade Ezzedine, Cécile Chardon, Stéphan Jacquet. New 16S rRNA primers to uncover Bdellovibrio and like organisms diversity and abundance. Journal of Microbiological Methods, Elsevier, 2020, ⟨10.1016/j.mimet.2020.105996⟩. ⟨hal-02935301⟩



Record views


Files downloads