, Food-grade sodium chloride was purchased from Xiangheng Co

, Liquid-liquid extraction (LLE) of volatile compounds in soy sauce An aliquot (50 mL) of soy sauce, added with 20 ?L internal standard (2-methyl-3-heptanone, 172.4 mg/L in methanol), was extracted by 50 mL dichloromethane three times. After 30 minutes of extraction, the solvent layer was separated by centrifugation (7012 g, 4 °C, 10 min). Then the organic extract was combined, dried over anhydrous sodium sulfate, and kept at -20 °C overnight

, filtered through 0.22 ?m organic membrane, and then concentrated to 1 mL by mild nitrogen stream, Jingke Instrument Factory

, Gas chromatography-mass spectrometry (GC-MS) analysis

, GC-MS analysis was performed by a Trace GC-MS system analysis

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T. Zhou, Experiment manipulation, Data analysis, Writing -original

Y. Feng, Writing -review & editing, Project administration

T. Thomas-danguin, Methodology, Revise

M. Zhao,