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Debunking the Myth of the Endogenous Antiangiogenic Vegfaxxxb Transcripts

Abstract : In this opinion article we critically assess evidence for the existence of a family of antiangiogenic vascular endothelial growth factor (Vegfaxxxb) transcripts, arising from the use of a phylogenetically conserved alternative distal splice site within exon 8 of the VEGFA gene. We explain that prior evidence for Vegfaxxxb transcripts in tissues rests heavily upon flawed RT-PCR methodologies, with the extensive use of 5'-tailing in primer design being the main issue. Furthermore, our analysis of large RNA-seq data sets (human and ovine) fails to identify a single Vegfaxxxb transcript. Therefore, we challenge the very existence of Vegfaxxxb transcripts, which further questions the physiological relevance of studies based on the use of 'anti-VEGFAxxxb' antibodies. Our analysis has implications for the proposed therapeutic use of isoform-specific anti-VEGFA strategies for treating cancer and retinopathies.
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https://hal.inrae.fr/hal-02927071
Contributor : Christopher Lallemant <>
Submitted on : Tuesday, September 1, 2020 - 1:08:33 PM
Last modification on : Wednesday, May 19, 2021 - 10:37:18 AM

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Hugues Dardente, William English, Manoj Valluru, Chryso Kanthou, David Simpson. Debunking the Myth of the Endogenous Antiangiogenic Vegfaxxxb Transcripts. Trends in Endocrinology and Metabolism = Trends in Endocrinology & Metabolism , Elsevier, 2020, 31 (6), pp.398-409. ⟨10.1016/j.tem.2020.01.014⟩. ⟨hal-02927071⟩

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