Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Postprandial NMR-Based Metabolic Exchanges Reflect Impaired Phenotypic Flexibility across Splanchnic Organs in the Obese Yucatan Mini-Pig

Abstract : The postprandial period represents one of the most challenging phenomena in whole-body metabolism, and it can be used as a unique window to evaluate the phenotypic flexibility of an individual in response to a given meal, which can be done by measuring the resilience of the metabolome. However, this exploration of the metabolism has never been applied to the arteriovenous (AV) exploration of organs metabolism. Here, we applied an AV metabolomics strategy to evaluate the postprandial flexibility across the liver and the intestine of mini-pigs subjected to a high fat-high sucrose (HFHS) diet for 2 months. We identified for the first time a postprandial signature associated to the insulin resistance and obesity outcomes, and we showed that the splanchnic postprandial metabolome was considerably affected by the meal and the obesity condition. Most of the changes induced by obesity were observed in the exchanges across the liver, where the metabolism was reorganized to maintain whole body glucose homeostasis by routing glucose formed de novo from a large variety of substrates into glycogen. Furthermore, metabolites related to lipid handling and energy metabolism showed a blunted postprandial response in the obese animals across organs. Finally, some of our results reflect a loss of flexibility in response to the HFHS meal challenge in unsuspected metabolic pathways that must be further explored as potential new events involved in early obesity and the onset of insulin resistance.
Type de document :
Article dans une revue
Liste complète des métadonnées

Littérature citée [55 références]  Voir  Masquer  Télécharger

https://hal.inrae.fr/hal-02927174
Déposant : Hélène Lesur <>
Soumis le : mardi 1 septembre 2020 - 14:35:15
Dernière modification le : jeudi 10 septembre 2020 - 17:06:04

Fichier

2020_nutrients_tremblay-franco...
Fichiers éditeurs autorisés sur une archive ouverte

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Citation

Marie Tremblay-Franco, Nathalie Poupin, Aurélien Amiel, Cécile Canlet, Didier Remond, et al.. Postprandial NMR-Based Metabolic Exchanges Reflect Impaired Phenotypic Flexibility across Splanchnic Organs in the Obese Yucatan Mini-Pig. Nutrients, MDPI, 2020, 12 (8), pp.2442. ⟨10.3390/nu12082442⟩. ⟨hal-02927174⟩

Partager

Métriques

Consultations de la notice

74

Téléchargements de fichiers

57