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Monitoring methionine sulfoxide with stereospecific mechanism-based fluorescent sensors

Abstract : Methionine can be reversibly oxidized to methionine sulfoxide (MetO) under physiological and pathophysiological conditions, but its use as a redox marker suffers from the lack of tools to detect and quantify MetO within cells. In this work, we created a pair of complementary stereospecific genetically encoded mechanism-based ratiometric fluorescent sensors of MetO by inserting a circularly permuted yellow fluorescent protein between yeast methionine sulfoxide reductases and thioredoxins. The two sensors, respectively named MetSOx and MetROx for their ability to detect S and R forms of MetO, were used for targeted analysis of protein oxidation, regulation and repair as well as for monitoring MetO in bacterial and mammalian cells, analyzing compartment-specific changes in MetO and examining responses to physiological stimuli.
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https://hal.inrae.fr/hal-02929705
Contributor : Lionel Tarrago <>
Submitted on : Thursday, September 3, 2020 - 4:42:39 PM
Last modification on : Thursday, December 3, 2020 - 4:58:02 PM

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Lionel Tarrago, Zalán Péterfi, Byung Cheon Lee, Thomas Michel, Vadim Gladyshev. Monitoring methionine sulfoxide with stereospecific mechanism-based fluorescent sensors. Nature Chemical Biology, Nature Publishing Group, 2015, 11 (5), pp.332-338. ⟨10.1038/nchembio.1787⟩. ⟨hal-02929705⟩

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