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Polymerase IV Plays a Crucial Role in Pollen Development in Capsella

Abstract : In Arabidopsis (Arabidopsis thaliana), DNA-dependent RNA polymerase IV (Pol IV) is required for the formation of transposable element (TE)-derived small RNA transcripts. These transcripts are processed by DICER-LIKE3 into 24-nucleotide small interfering RNAs (siRNAs) that guide RNA-directed DNA methylation. In the pollen grain, Pol IV is also required for the accumulation of 21/22-nucleotide epigenetically activated siRNAs, which likely silence TEs via post-transcriptional mechanisms. Despite this proposed role of Pol IV, its loss of function in Arabidopsis does not cause a discernible pollen defect. Here, we show that the knockout of NRPD1, encoding the largest subunit of Pol IV, in the Brassicaceae species Capsella (Capsella rubella), caused postmeiotic arrest of pollen development at the microspore stage. As in Arabidopsis, all TE-derived siRNAs were depleted in Capsella nrpd1 microspores. In the wild-type background, the same TEs produced 21/22-nucleotide and 24-nucleotide siRNAs; these processes required Pol IV activity. Arrest of Capsella nrpd1 microspores was accompanied by the deregulation of genes targeted by Pol IV-dependent siRNAs. TEs were much closer to genes in Capsella compared with Arabidopsis, perhaps explaining the essential role of Pol IV in pollen development in Capsella. Our discovery that Pol IV is functionally required in Capsella microspores emphasizes the relevance of investigating different plant models.
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https://hal.inrae.fr/hal-02935298
Contributor : Christopher Lallemant <>
Submitted on : Thursday, September 10, 2020 - 11:27:09 AM
Last modification on : Monday, November 23, 2020 - 3:06:04 PM

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Zhenxing Wang, Nicolas Butel, Juan Santos-González, Filipe Borges, Jun Yi, et al.. Polymerase IV Plays a Crucial Role in Pollen Development in Capsella. The Plant cell, American Society of Plant Biologists (ASPB), 2020, 32 (4), pp.950-966. ⟨10.1105/tpc.19.00938⟩. ⟨hal-02935298⟩

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