Determinants of activity in glutaredoxins: an in vitro evolved Grx1-like variant of Escherichia coli Grx3 - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Biochemical Journal Année : 2010

Determinants of activity in glutaredoxins: an in vitro evolved Grx1-like variant of Escherichia coli Grx3

Tobias h. Elgán
  • Fonction : Auteur
Jon Beckwith
  • Fonction : Auteur
Peter Güntert
  • Fonction : Auteur
Kurt d. Berndt
  • Fonction : Auteur

Résumé

The Escherichia coli glutaredox ins 1 and 3 (Grx 1 and Grx3) are structurally similar (37 % sequence identity), yet have different activities in vivo. Unlike Grx3, Grx 1 efficiently reduces protein disulfides in proteins such as RR (ribonucleotide reductase), whereas it is poor at reducing S-glutathionylated proteins. An E. coli strain lacking genes encoding thioredoxins 1 and 2 and Grx 1 is not viable on either rich or minimal medium; however, a M43V mutation in Grx3 restores growth under these conditions and results in a Grx1-like protein [Ortenberg, Con, Porat and Beckwith (2004) Proc. Natl. Acad. Sci. U.S.A. 101, 7439-7944]. To uncover the structural basis of this change in activity, we have compared wild-type and mutant Grx3 using CD and NMR spectroscopy. Ligand-induced stability measurements demonstrate that the Grx3(M43V/C65Y) mutant has acquired affinity for RR. Far-UV CD spectra reveal no significant differences, but differences are observed in the near-UV region indicative of tertiary structural changes. NMR H-1-N-15 HSQC (heteronuclear single quantum coherence) spectra show that approximately half of the 82 residues experience significant (Delta delta > 0.03 p.p.m.) chemical shift deviations in the mutant, including nine residues experiencing extensive (Delta delta >= 0.15 p.p.m.) deviations. To test whether the M43V mutation alters dynamic properties of Grx3, H/D (hydrogen/deuterium) exchange experiments were performed demonstrating that the rate at which backbone amides exchange protons with the solvent is dramatically enhanced in the mutant, particularly in the core of the protein. These data suggest that the Grx1-like activity of the Grx3(M43V/C65Y) mutant may be explained by enhanced intrinsic motion allowing for increased specificity towards larger substrates such as RR.

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hal-02941413 , version 1 (17-09-2020)

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Tobias h. Elgán, Anne-Gaëlle Planson, Jon Beckwith, Peter Güntert, Kurt d. Berndt. Determinants of activity in glutaredoxins: an in vitro evolved Grx1-like variant of Escherichia coli Grx3. Biochemical Journal, 2010, 430 (3), pp.487-495. ⟨10.1042/BJ20100289⟩. ⟨hal-02941413⟩
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