Optimization of permeabilized fibers preparation for mitochondrial respiration measurements using Design of Experiments methodology. - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Conference Poster Year : 2017

Optimization of permeabilized fibers preparation for mitochondrial respiration measurements using Design of Experiments methodology.

Fabienne Cortade
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Béatrice Chabi

Abstract

To optimize the permeabilized fibers (pf) preparation from mouse Tibialis anterior in our lab, we used the Design of Experiments (DoE) methodology that evaluates the impact of 6 experimental conditions or factors, on the pf respiration parameters (Pyruvate Malate Succinate respiration (PMS leak) and respiratory control ratio (RCRPMS)), to provide a maximum of information using a limited number of experiments and animals.
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Dates and versions

hal-02941628 , version 1 (17-09-2020)

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  • HAL Id : hal-02941628 , version 1

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Fabienne Cortade, Virginie Gaillard, Béatrice Chabi. Optimization of permeabilized fibers preparation for mitochondrial respiration measurements using Design of Experiments methodology.. MITOEAGLE 2017, Jul 2017, Obergurgl, Austria. , 2017. ⟨hal-02941628⟩
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