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 Optimization of permeabilized fibers preparation for mitochondrial respiration measurements using Design of Experiments methodology. Fabienne Cortade 36f149ad3e0d6e6f3f71c0d4216f39bd inra.fr 1205661 247727-1205661 Virginie Gaillard 557711-0 Béatrice Chabi daac551defd5679b0e8a2816c0743488 inrae.fr beatrice-chabi 736660 50797-736660 https://orcid.org/0000-0002-5604-5668 https://www.idref.fr/077823672 Christelle Raynaud ddfeb536593241d55e60e601feaa33e7 inrae.fr 2020-09-17 10:54:03 2025-03-21 15:12:21 2020-09-17 10:55:00 2017-07-28 2020-09-17 Scientists contributor Christelle Raynaud ddfeb536593241d55e60e601feaa33e7 inrae.fr CCSD hal-02941628 https://hal.inrae.fr/hal-02941628 cortade:hal-02941628 <i>MITOEAGLE 2017</i>, Jul 2017, Obergurgl, Austria. <a target="_blank" href="https://wiki.oroboros.at/index.php/MitoEAGLE_Obergurgl_2017"></a>, 2017 MITOEAGLE 2017, Jul 2017, Obergurgl, Austria. , 2017 HAL Authorization INRA - Institut national de la recherche agronomique Archive ouverte en agrobiosciences Biologie-Santé Université de Montpellier Dynamique Musculaire Et Métabolisme Institut National de Recherche en Agriculture, Alimentation et Environnement INRAE Occitanie Montpellier Physiologie animale et systèmes d’élevage Université de Montpellier (2015-2021) International No No Yes No Optimization of permeabilized fibers preparation for mitochondrial respiration measurements using Design of Experiments methodology. Fabienne Cortade 36f149ad3e0d6e6f3f71c0d4216f39bd inra.fr 1205661 247727-1205661 Virginie Gaillard 557711-0 Béatrice Chabi daac551defd5679b0e8a2816c0743488 inrae.fr beatrice-chabi 736660 50797-736660 https://orcid.org/0000-0002-5604-5668 https://www.idref.fr/077823672 MITOEAGLE 2017 2017-07-28 2017-07-29 Obergurgl Austria 2017 https://wiki.oroboros.at/index.php/MitoEAGLE_Obergurgl_2017 English Life Sciences [q-bio] Conference poster Conference poster Conference poster

To optimize the permeabilized fibers (pf) preparation from mouse Tibialis anterior in our lab, we used the Design of Experiments (DoE) methodology thatevaluates the impact of 6 experimental conditions or factors, on the pf respiration parameters (Pyruvate Malate Succinate respiration (PMS leak) andrespiratory control ratio (RCRPMS)), to provide a maximum of information using a limited number of experiments and animals.

 199917397U Dynamique Musculaire et Métabolisme DMEM 2011-01-01 2019-12-31
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