, TNF-?, and IL-17A producing CD2 + and CD2 ? ?? T cells in the presence of DON and DOM-1. Violet proliferation dye-stained PBMCs were cultivated for 4 days in the presence of ConA alone or in combination, FIGURE 9 | Frequencies of IFN-?
A) CD2 + and CD2 ? ?? T cells were gated and further analyzed for production of TNF-?, IFN-?, and IL-17A in the presence of DON and DOM-1. The black rectangles in the pseudocolor plots show the gating strategy that was applied for analysis of total IFN-?, total TNF-?, and total IL-17A producing cells. Red numbers give percentages of cytokine producing ?? T cells in these gates. Representative flow cytometry data from one animal is shown. (B,C) Boxplots show the results for ?? T cells within PBMCs from six individual pigs. (B) Frequency of IFN-? and TNF-? producing cells within CD2 + ?? T cells. (C) Frequency of IFN-? ,
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