Inducing Sequential Cycles of Epithelial-Mesenchymal and Mesenchymal-Epithelial Transitions in Mammary Epithelial Cells

Epithelial-Mesenchymal Transition (EMT) and its reciprocal Mesenchymal-Epithelial Transition (MET) occur naturally as a cycling process during embryonic and foetal development. The capacity of such iterative cycles to drive cell fate and cellular and molecular behaviour in physiology or pathology remains unclear. We describe here a protocol to induce successive cycles of EMT/MET in an untransformed human mammary epithelial cell line (MCF10A) as well as the necessary controls for cycle validation.

Mots clés

Cycles EMT Epithelial-mesenchymal transition MCF10A MET Mesenchymal-epithelial transition.

Domaines

Interactions cellulaires [q-bio.CB] Biologie cellulaire

Hervé Acloque : Connectez-vous pour contacter le contributeur

https://hal.inrae.fr/hal-02950184

Soumis le : dimanche 27 septembre 2020-14:48:06

Dernière modification le : samedi 6 avril 2024-10:20:07

Dates et versions

hal-02950184 , version 1 (27-09-2020)

Identifiants

HAL Id : hal-02950184 , version 1
DOI : 10.1007/978-1-0716-0779-4_26
PUBMED : 32939732
WOS : 000706803900027

Citer

Cecile Davaine, Eva Hadadi, William Taylor, Annelise Bennaceur-Griscelli, Hervé Acloque. Inducing Sequential Cycles of Epithelial-Mesenchymal and Mesenchymal-Epithelial Transitions in Mammary Epithelial Cells. The Epithelial-to Mesenchymal Transition, pp.341-351, 2021, ⟨10.1007/978-1-0716-0779-4_26⟩. ⟨hal-02950184⟩

Exporter

BibTeX XML-TEI Dublin Core DC Terms EndNote DataCite

Collections

AGROPARISTECH UNIV-PARIS-SACLAY INRAE GENETIQUE_ANIMALE GS-BIOSPHERA GS-LIFE-SCIENCES-HEALTH GABI ONCOSTEM SAPS

36 Consultations

0 Téléchargements