Skip to Main content Skip to Navigation
Journal articles

D-Tailor: automated analysis and design of DNA sequences

Abstract : Motivation: Current advances in DNA synthesis, cloning and sequencing technologies afford high throughput implementation of artificial sequences into living cells. However, flexible computational tools for multi-objective sequence design are lacking, limiting the potential of these technologies. Results: We developed DNA-Tailor (D-Tailor), a fully extendable software framework, for property-based design of synthetic DNA sequences. D-Tailor permits the seamless integration of multiple sequence analysis tools into a generic Monte-Carlo simulation that evolves sequences toward any combination of rationally defined properties. As proof of principle, we show that D-Tailor is capable of designing sequence libraries comprising all possible combinations among three different sequence properties influencing translation efficiency in E. coli. The capacity to design artificial sequences that systematically sample any given parameter space should support the implementation of more rigorous experimental designs. Availability: Source code is available for download at https://sourceforge.net/projects/dtailor/
Complete list of metadata

Cited literature [52 references]  Display  Hide  Download

https://hal.inrae.fr/hal-02950423
Contributor : Guillaume Cambray <>
Submitted on : Sunday, September 27, 2020 - 11:28:55 PM
Last modification on : Tuesday, October 13, 2020 - 3:35:59 AM

File

2014.bionfinformatics_w_sup.pd...
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Joao Guimarães, Miguel Rocha, Adam Arkin, Guillaume Cambray. D-Tailor: automated analysis and design of DNA sequences. Bioinformatics, Oxford University Press (OUP), 2014, 30 (8), pp.1087-1094. ⟨10.1093/bioinformatics/btt742⟩. ⟨hal-02950423⟩

Share

Metrics

Record views

24

Files downloads

49