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Master thesis

Étude génoprotéomique de régulateurs potentiels de la lipoprotéine lipase (LPL) mammaire dans le lait : impact sur sa fonction biologique in vitro

Abstract : Lipolysis is an enzymatic reaction that adversely affects the organoleptic and technological qualities of milk. It is mostly due to the action of lipoprotein lipase (LPL) on the milk fat globules. The biochemical basis of lipolysis is related to the balance between activation and inhibition of LPL (post-translational regulation). But it can also be linked to the post-transcriptional regulation of this enzyme. A thorough understanding of the regulation of LPL is then necessary to control lipolysis in milk. Therefore, we tried to characterize the 3'UTR region of the "lpl" gene in the mammary gland and in mammary epithelial cells isolated from milk. We demonstrated that two LPL transcripts exist in the mammary gland whereas only one can be characterized in isolated mammary epithelial cells. We also searched for protein / peptide cofactors of LPL in milk by liquid chromatography coupled with mass spectrometry (LC / MS). Finally, our study shows a positive correlation between the level of proteolysis and that of lipolysis in milk. We therefore suggest that the natural proteolysis products of milk have an activating role of LPL.
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https://hal.inrae.fr/hal-02954875
Contributor : Christelle Cebo <>
Submitted on : Thursday, October 1, 2020 - 1:51:42 PM
Last modification on : Tuesday, June 15, 2021 - 2:57:32 PM
Long-term archiving on: : Monday, January 4, 2021 - 8:51:23 AM

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Christelle Cebo, Sylia Goudjil. Étude génoprotéomique de régulateurs potentiels de la lipoprotéine lipase (LPL) mammaire dans le lait : impact sur sa fonction biologique in vitro. Sciences du Vivant [q-bio]. 2017. ⟨hal-02954875⟩

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