Skip to Main content Skip to Navigation
Journal articles

Molecular Characterization of the Complete Coding Sequence of Olive Leaf Yellowing-Associated Virus

Abstract : Genome organization and phylogenetic relationships of olive leaf yellowing-associated virus (OLYaV) with other members of the Closteroviridae family were determined. The complete coding sequence of OLYaV was obtained by high throughput sequencing of total RNA from a 35-year-old olive tree (cv. Zarzaleña) from Brazil, showing olive leaf yellowing disease and deformations in the wood. This represents the first report of OLYaV in this country. A genomic sequence of 16,700 nt containing 11 open reading frames (ORFs) was recovered, representing the complete virus coding capacity. The knowledge of the nucleotide sequence of the genome including the gene that codes the coat protein will facilitate the development of diagnostic tests, which are limited so far to PCR-based methods targeting the HSP70h gene. Interestingly, a thaumatin-like protein (ORF2), previously reported in other unassigned viruses in the Closteroviridae family, persimmon virus B and actidinia virus 1, was identified in the OLYaV genome. Phylogenetic analysis of shared proteins (ORF1a, ORF1b, HSP70h, HSP90h and CP) with all members of the Closteroviridae family provides new insight into the taxonomic position of these three closteroviruses and suggests they could represent a new genus in the family.
Complete list of metadata

https://hal.inrae.fr/hal-02955256
Contributor : Muriel Gauthier <>
Submitted on : Thursday, October 1, 2020 - 4:25:00 PM
Last modification on : Monday, June 21, 2021 - 11:46:10 AM

File

2020-Ruiz-Garcia_Plants.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Ana Belén Ruiz-García, Thierry Candresse, Celia Canales, Félix Morán, Carlos Machado de Oliveira, et al.. Molecular Characterization of the Complete Coding Sequence of Olive Leaf Yellowing-Associated Virus. Plants, MDPI, 2020, 9 (10), pp.1272. ⟨10.3390/plants9101272⟩. ⟨hal-02955256⟩

Share

Metrics

Record views

12

Files downloads

21