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Inward and outward rectifying potassium currents in Saccharomyces-Cerevisiae mediated by endogenous and heterelogously expressed ion channels

Abstract : Disruption of genes encoding endogenous transport proteins in Saccharomyces cerevisiae has facilitated the recent cloning, by functional expression, of cDNAs encoding K+ channels and amino acid transporters front the plant Arabidopsis thaliana [1-4]. In the present study, we demonstrate in whole-cell patch clamp experiments that the inability of trk1 Delta trk2 Delta mutants of S. cervisiae to grow on submillimolar K+ correlates with the lack of K+ inward currents, which are present in wild-type cells, and that transformation of the trk1 Delta trk2 Delta double-deletion mutant with KAT1 from Arabidopsis thaliana restores this phenotype hy encoding a plasma membrane protein that allows large K+ inward currents. Similar K+ inward currents are induced by transformation of a trk1 mutant with AKT1 from A. thaliana.
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https://hal.inrae.fr/hal-02965870
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Submitted on : Tuesday, October 13, 2020 - 3:42:11 PM
Last modification on : Friday, March 5, 2021 - 3:30:16 AM

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A. Bertl, J. Anderson, C. Slayman, Hervé Sentenac, R. Gaber. Inward and outward rectifying potassium currents in Saccharomyces-Cerevisiae mediated by endogenous and heterelogously expressed ion channels. Folia Microbiologica, Springer Verlag (Germany), 1994, 39 (6), pp.507-509. ⟨10.1007/BF02814074⟩. ⟨hal-02965870⟩

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