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Bioanalytical characterisation of multiple endocrine- and dioxin-like activities in sediments from reference and impacted small rivers

Abstract : A comprehensive evaluation of organic contamination was performed in sediments sampled in tworeference and three impacted small streams where endocrine disruptive (ED) effects in fish have beenevidenced. The approach combined quantitative chemical analyses of more than 50 ED chemicals (EDCs)and a battery ofin vitrobioassays allowing the quantification of receptor-mediated activities, namelyestrogen (ER), androgen (AR), dioxin (AhR) and pregnane X (PXR) receptors. At the most impacted sites,chemical analyses showed the presence of natural estrogens, organochlorine pesticides, parabens,polycyclic aromatic hydrocarbons (16 PAHs), bisphenol A and alkylphenols, while synthetic steroids,myco-estrogens and phyto-estrogens were not detected. Determination of toxic-equivalent amountsshowed that 28–96% of estrogenic activities in bioassays (0.2–6.3 ng/g 17b-estradiol equivalents) wereexplained by 17b-estradiol and estrone. PAHs were major contributors (20–60%) to the total dioxin-likeactivities. Interestingly, high PXR and (anti)AR activities were detected; however, the targeted analysedcompounds could not explain the measured biological activities. This study highlighted the presence ofmultiple organic EDCs in French river sediments subjected to mixed diffuse pollution, and argues for theneed to further identify AR and PXR active compounds in the aquatic environment.
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https://hal.inrae.fr/hal-02972431
Contributor : Nicolas Creusot <>
Submitted on : Tuesday, October 20, 2020 - 1:32:28 PM
Last modification on : Wednesday, October 21, 2020 - 11:38:29 AM

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Said Kinani, Stéphane Bouchonnet, Nicolas Creusot, Sophie Bourcier, Patrick Balaguer, et al.. Bioanalytical characterisation of multiple endocrine- and dioxin-like activities in sediments from reference and impacted small rivers. Environmental Pollution, Elsevier, 2010, 158 (1), pp.74-83. ⟨10.1016/j.envpol.2009.07.041⟩. ⟨hal-02972431⟩

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