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Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results

Peter Møller 1 Amaya Azqueta 2, 3 Elisa Boutet-Robinet 4 Gudrun Koppen 5 Stefano Bonassi 6, 7 Mirta Milić 8 Goran Gajski 8 Solange Costa 9, 10 João Paulo Teixeira 9, 10 Cristiana Costa Pereira 9, 10 Maria Dusinska 11 Roger Godschalk 12 Gunnar Brunborg 13 Kristine Gutzkow 13 Lisa Giovannelli 14 Marcus Cooke 15 Elke Richling 16 Blanca Laffon 17, 18 Vanessa Valdiglesias 17, 18 Nursen Basaran 19 Cristian del Bo’ 20 Bojana Zegura 21 Matjaz Novak 21 Helga Stopper 22 Pavel Vodicka 23, 24 Sona Vodenkova 24, 23 Vanessa Moraes de Andrade 25 Monika Sramkova 26 Alena Gabelova 26 Andrew Collins 27 Sabine Langie 12, 28
Abstract : The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between 'desirable' and 'essential' information: 'essential' information refers to the precise details that are necessary to assess the quality of the experimental work, whereas 'desirable' information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.
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Submitted on : Tuesday, November 3, 2020 - 3:00:58 PM
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Peter Møller, Amaya Azqueta, Elisa Boutet-Robinet, Gudrun Koppen, Stefano Bonassi, et al.. Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results. Nature Protocols, Nature Publishing Group, 2020, ⟨10.1038/s41596-020-0398-1⟩. ⟨hal-02987047⟩



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