Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results
Peter Møller
(1)
,
Amaya Azqueta
(2, 3)
,
Elisa Boutet-Robinet
(4)
,
Gudrun Koppen
(5)
,
Stefano Bonassi
(6, 7)
,
Mirta Milić
(8)
,
Goran Gajski
(8)
,
Solange Costa
(9, 10)
,
João Paulo Teixeira
(9, 10)
,
Cristiana Costa Pereira
(9, 10)
,
Maria Dusinska
(11)
,
Roger Godschalk
(12)
,
Gunnar Brunborg
(13)
,
Kristine B Gutzkow
(13)
,
Lisa Giovannelli
(14)
,
Marcus S Cooke
(15)
,
Elke Richling
(16)
,
Blanca Laffon
(17, 18)
,
Vanessa Valdiglesias
(17, 18)
,
Nursen Basaran
(19)
,
Cristian del Bo’
(20)
,
Bojana Zegura
(21)
,
Matjaz Novak
(21)
,
Helga Stopper
(22)
,
Pavel Vodicka
(23, 24)
,
Sona Vodenkova
(24, 23)
,
Vanessa Moraes de Andrade
(25)
,
Monika Sramkova
(26)
,
Alena Gabelova
(26)
,
Andrew Collins
(27)
,
Sabine a S Langie
(12, 28)
1
UCPH -
University of Copenhagen = Københavns Universitet
2 UNAV - Universidad de Navarra [Pamplona]
3 IdiSNA - Navarra Institute for Health Research / Instituto de Investigación Sanitaria de Navarra
4 ToxAlim-COMICS - Contaminants & Stress Cellulaire
5 VITO - Flemish Institute for Technological Research
6 IRCCS San Raffaele Pisana - Istituto di Ricovero e Cura a Carattere Scientifico
7 UniSR - Universita Vita Salute San Raffaele = Vita-Salute San Raffaele University [Milan, Italie]
8 Institute for Medical Research and Occupational Health
9 INSA - Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal]
10 Universidade do Porto = University of Porto
11 NILU - Norwegian Institute for Air Research
12 NUTRIM - School of Nutrition and Translational Research in Metabolism [Maastricht]
13 NIPH - Norwegian Institute of Public Health [Oslo]
14 NEUROFARBA Department [Firenze, Italy]
15 USF - University of South Florida [Tampa]
16 Department of Chemistry - Food Chemistry and Toxicology [Kaiserslautern, Germany]
17 Universidade da Coruña
18 INIBIC - Instituto de Investigación Biomédica de A Coruña [La Corogne, Espagne]
19 Hacettepe University = Hacettepe Üniversitesi
20 UNIMI - Università degli Studi di Milano = University of Milan
21 NIB - National Institute of Biology [Ljubljana]
22 University of Würzburg
23 CAS - Czech Academy of Sciences [Prague]
24 UK - Univerzita Karlova [Praha, Česká republika] = Charles University [Prague, Czech Republic]
25 UNISUL - University of Southern Santa Catarina [Florianopolis]
26 SAS - Slovak Academy of Sciences
27 UiO - University of Oslo
28 UHasselt - Hasselt University
2 UNAV - Universidad de Navarra [Pamplona]
3 IdiSNA - Navarra Institute for Health Research / Instituto de Investigación Sanitaria de Navarra
4 ToxAlim-COMICS - Contaminants & Stress Cellulaire
5 VITO - Flemish Institute for Technological Research
6 IRCCS San Raffaele Pisana - Istituto di Ricovero e Cura a Carattere Scientifico
7 UniSR - Universita Vita Salute San Raffaele = Vita-Salute San Raffaele University [Milan, Italie]
8 Institute for Medical Research and Occupational Health
9 INSA - Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal]
10 Universidade do Porto = University of Porto
11 NILU - Norwegian Institute for Air Research
12 NUTRIM - School of Nutrition and Translational Research in Metabolism [Maastricht]
13 NIPH - Norwegian Institute of Public Health [Oslo]
14 NEUROFARBA Department [Firenze, Italy]
15 USF - University of South Florida [Tampa]
16 Department of Chemistry - Food Chemistry and Toxicology [Kaiserslautern, Germany]
17 Universidade da Coruña
18 INIBIC - Instituto de Investigación Biomédica de A Coruña [La Corogne, Espagne]
19 Hacettepe University = Hacettepe Üniversitesi
20 UNIMI - Università degli Studi di Milano = University of Milan
21 NIB - National Institute of Biology [Ljubljana]
22 University of Würzburg
23 CAS - Czech Academy of Sciences [Prague]
24 UK - Univerzita Karlova [Praha, Česká republika] = Charles University [Prague, Czech Republic]
25 UNISUL - University of Southern Santa Catarina [Florianopolis]
26 SAS - Slovak Academy of Sciences
27 UiO - University of Oslo
28 UHasselt - Hasselt University
Elisa Boutet-Robinet
- Fonction : Auteur
- PersonId : 746889
- IdHAL : elisa-boutet-robinet
- ORCID : 0000-0002-5223-4568
- IdRef : 071622950
Résumé
The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between 'desirable' and 'essential' information: 'essential' information refers to the precise details that are necessary to assess the quality of the experimental work, whereas 'desirable' information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.
Domaines
Sciences du Vivant [q-bio]
Origine : Fichiers éditeurs autorisés sur une archive ouverte
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