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Cloning and Partial Characterization of an Extracellular Dextransucrase Coding Region (DSR-V) from Leuconostoc citreum M-3

Abstract : The dextransucrase enzymes synthesize dextran, a glucose polymer with broad industrial applications, making the search for new dextransucrases of great interest. The work described aimed at the partial characterizing of a recombinant dextransucrase enzyme from Leuconostoc citreum M-3. From the genomic DNA of strain M-3, an amplicon containing a coding region of a dextransucrase called DSRV was isolated, and deposited in the GenBankTM (Accession number: KF724950). The amino acid sequence alignment of DSR-V with other dextransucrases demonstrated that it shares a 94% identity with the DSR-D of L. mesenteroides Lcc4 and the DSR-S of L. mesenteroides NRRL B-512F. The DSR-V was cloned and expressed in Escherichia coli JM109 facilitating the formation and detection of DSR-V specific dextran. The SDS-PAGE soluble fraction zymography of E. coli DSR-V and the C-13-NMR spectra of dextran polymers synthesized by this clone confirm that L. citreum M-3 dsrV gene codes for a different dextransucrase synthesizing linear dextrans with mainly alpha(1-6) linkages.
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https://hal.inrae.fr/hal-03030297
Contributor : Dominique Fournier <>
Submitted on : Monday, November 30, 2020 - 7:51:53 AM
Last modification on : Thursday, May 13, 2021 - 3:41:52 AM

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Reinaldo Vidal, Sandra Pacios Michelena, Roberto C Aristicas Ribalta, Lisandra Martínez Valdés, Meinardo Lafargue Gámez, et al.. Cloning and Partial Characterization of an Extracellular Dextransucrase Coding Region (DSR-V) from Leuconostoc citreum M-3. Özlem Ateş Duru. Microbial Exopolysaccharides: Current Research and Developments, Caister Academic Press, 2019, 978-1-912530-27-4; 978-1-912530-26-7. ⟨10.21775/9781912530267.11⟩. ⟨hal-03030297⟩

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