Skip to Main content Skip to Navigation
Journal articles

Thermotolerance, Survival, and Stability of Lactic Acid Bacteria After Spray Drying as Affected by the Increase of Growth Temperature

Abstract : Microencapsulation of lactic acid bacteria (LAB) via spray drying differs from that of common bioactive substances in that the intrinsic stress tolerance of cells can be modulated to improve cell survival. In this study, elevated growth temperatures that were 3–5 °C above the standard conditions were used to culture Lactococcus lactis subsp. cremoris, Lactobacillus rhamnosus GG (LGG), and Lactobacillus acidophilus for spray drying. The heat-adapted cultures showed lower bacterial population than the controls by 0.45 log at stationary growth phase and produced lactobacilli cells with elongated shape, while their metabolic activities were maintained similar to the controls. Heat-adapted L. cremoris and LGG demonstrated increases in survival by 0.7– 1.5 log and 0.3 log, respectively, after heat treatment at 60 °C. The thermotolerance of L. acidophilus grown at 42 °C was dependent on growth phase, and the culture entered death phase within 24 h of incubation. The survival of heat-adapted L.cremoris and L. acidophilus after spray drying was increased by 21.0% and 13.7%, respectively, whereas the increase shown by LGG was relatively insignificant (9.9%). Spray-dried powders containing heat-adapted cells showed substantial reduction of viability at the first week of storage, reaching 1.03–1.23 log, compared to 0.87–0.90 log of reduction shown by the controls. The findings demonstrated that strain-specific cellular response toward variations in growth conditions is crucial to the intrinsic properties of LAB and to cell survival during spray drying and storage. Controlling cellular response is one of the key factors in developing a viable spray drying scheme for active LAB.
Complete list of metadata

https://hal.inrae.fr/hal-03106934
Contributor : Anne Giboulot <>
Submitted on : Tuesday, January 12, 2021 - 11:27:37 AM
Last modification on : Tuesday, April 13, 2021 - 3:33:39 AM

File

Hao2021_Article_Thermotoleranc...
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Fan Hao, Nan Fu, Hamadel Ndiaye, Meng Wai Woo, Romain Jeantet, et al.. Thermotolerance, Survival, and Stability of Lactic Acid Bacteria After Spray Drying as Affected by the Increase of Growth Temperature. Food and Bioprocess Technology, Springer, 2021, pp.6. ⟨10.3390/foods10010006⟩. ⟨hal-03106934⟩

Share

Metrics

Record views

51

Files downloads

100