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Differential contribution of two organelles of endosymbiotic origin to iron-sulfur cluster synthesis in Toxoplasma

Abstract : Iron-sulfur (Fe-S) clusters are one of the most ancient and ubiquitous prosthetic groups, and they are required by a variety of proteins involved in important metabolic processes. Apicomplexan parasites have inherited different plastidic and mitochondrial Fe-S clusters biosynthesis pathways through endosymbiosis. We have investigated the relative contributions of these pathways to the fitness of Toxoplasma gondii, an apicomplexan parasite causing disease in humans, by generating specific mutants. Phenotypic analysis and quantitative proteomics allowed us to highlight striking differences in these mutants. Both Fe-S cluster synthesis pathways are necessary for optimal parasite growth in vitro, but their disruption leads to markedly different fates: impairment of the plastidic pathway leads to a loss of the organelle and to parasite death, while disruption of the mitochondrial pathway trigger differentiation into a stress resistance stage. This highlights that otherwise similar biochemical pathways hosted by different sub-cellular compartments can have very different contributions to the biology of the parasites, which is something to consider when exploring novel strategies for therapeutic intervention.
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Preprints, Working Papers, ...
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Submitted on : Friday, January 29, 2021 - 10:43:07 AM
Last modification on : Thursday, May 19, 2022 - 3:56:01 PM


PamukcuS.-et al-bioRxiv-2021.p...
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Sarah Pamukcu, Aude Cerutti, Sonia Hem, Valerie Rofidal, Sébastien Besteiro. Differential contribution of two organelles of endosymbiotic origin to iron-sulfur cluster synthesis in Toxoplasma. 2021. ⟨hal-03125013⟩



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