Skip to Main content Skip to Navigation
Journal articles

Intergenerationally Maintained Histone H4 Lysine 16 Acetylation Is Instructive for Future Gene Activation

Abstract : Before zygotic genome activation (ZGA), the quiescent genome undergoes reprogramming to transition into the transcriptionally active state. However, the mechanisms underlying euchromatin establishment during early embryogenesis remain poorly understood. Here, we show that histone H4 lysine 16 acetylation (H4K16ac) is maintained from oocytes to fertilized embryos in Drosophila and mammals. H4K16ac forms large domains that control nucleosome accessibility of promoters prior to ZGA in flies. Maternal depletion of MOF acetyltransferase leading to H4K16ac loss causes aberrant RNA Pol II recruitment, compromises the 3D organization of the active genomic compartments during ZGA, and causes downregulation of post-zygotically expressed genes. Germline depletion of histone deacetylases revealed that other acetyl marks cannot compensate for H4K16ac loss in the oocyte. Moreover, zygotic re-expression of MOF was neither able to restore embryonic viability nor onset of X chromosome dosage compensation. Thus, maternal H4K16ac provides an instructive function to the offspring, priming future gene activation.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-03141899
Contributor : Anne-Sophie Grenier <>
Submitted on : Monday, February 15, 2021 - 3:53:17 PM
Last modification on : Friday, April 2, 2021 - 4:23:15 PM

Identifiers

Citation

Maria Samata, Anastasios Alexiadis, Gautier Richard, Plamen Georgiev, Johannes Nuebler, et al.. Intergenerationally Maintained Histone H4 Lysine 16 Acetylation Is Instructive for Future Gene Activation. Cell, Elsevier, 2020, 182 (1), pp.127-144.e23. ⟨10.1016/j.cell.2020.05.026⟩. ⟨hal-03141899⟩

Share

Metrics

Record views

13