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Multicolored Fluorescent In Situ Hybridization to Assess Pairing Configurations at Metaphase I in Brassica Hybrids

Abstract : Genetic diversity can be introduced into polyploid crop species through meiotic recombination by exchanges between homologous or homoeologous chromosomes. Fluorescent in situ hybridization (FISH) enables the characterization of these homologous and homoeologous chromosome pairs during meiosis and identification of structural rearrangements during mitosis in metaphase I. In this chapter, we describe a protocol for the multicolored fluorescent labeling of chromosome spreads. This protocol allows the characterization of each A and C homoeologous subgenomes in a polyploid species using a genome-specific BAC combined with specific chromosome labeling BAC sequences.
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https://hal.inrae.fr/hal-03144030
Contributor : Anne-Sophie Grenier <>
Submitted on : Wednesday, February 17, 2021 - 11:35:00 AM
Last modification on : Friday, April 2, 2021 - 4:21:06 PM

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Virginie Huteau, Olivier Coriton. Multicolored Fluorescent In Situ Hybridization to Assess Pairing Configurations at Metaphase I in Brassica Hybrids. HUMANA PRESS INC. Plant Meiosis: Methods And Protocols, 2061, 2020, Methods in Molecular Biology, 978-1-4939-9818-0. ⟨10.1007/978-1-4939-9818-0_12⟩. ⟨hal-03144030⟩

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