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Contribution of a molecular test for the diagnosis of genital infection with Trichomonas vaginalis and Mycoplasma genitalium

Abstract : In the present study, we assessed a recently-marketed molecular test, the S-DiaMGTV (TM) kit (Diagenode), which provides simultaneous detection of Mycoplasma genitalium and Trichomonas vaginalis in urogenital samples. Performance characteristics of the S-DiaMGTV (TM) kit were compared to an in-house PCR for detection of M. genitalium and, for first time, with direct observation of genital secretions in wet mounting microscopy for T. vaginalis, a routine laboratory method. For M. genitalium, out of 66 samples, two negative with the in-house PCR were found positive with the S-DiaMGTV (TM) kit and two positive with the in-house PCR were found negative with the kit. For T. vaginalis, four samples were found positive by the molecular test. Among them, two were previously tested by the wet mounting observation and only one was positive. The kit allows an increase of T. vaginalis detection even in a low incidence country. Performances of the kit are in favor of its use in routine laboratory practice.
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https://hal.inrae.fr/hal-03146855
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Submitted on : Friday, February 19, 2021 - 1:18:14 PM
Last modification on : Thursday, May 6, 2021 - 2:22:33 PM

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Adélaïde Chesnay, Benoit Lancelin, Cécile Le Brun, Adeline Pastuszka, Guillaume Desoubeaux, et al.. Contribution of a molecular test for the diagnosis of genital infection with Trichomonas vaginalis and Mycoplasma genitalium. Annales de Biologie Clinique, John Libbey Eurotext, 2020, 78 (6), pp.623-627. ⟨10.1684/abc.2020.1589⟩. ⟨hal-03146855⟩

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