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Analyse in vivo des capacités myogéniques des cellules souches musculaires par transplantation chez la truite arc-en-ciel (Oncorhynchus mykiss)

Abstract : Trout muscle has a high muscle growth capacity; involving muscle stem cells also called satellite cells. After differentiation and proliferation of these cells, the obtained myocytes will be able to fuse, either with existing fibers (hypertrophy), or between them to form new muscle fibers (hyperplasia). Hyperplasia declines with age and stops at sexual maturity (1.5-2kg). Transplantation was done with satellite cells of donors of different weights (100g and 500g) expressing a fluorescent protein (GFP) to determine if stem cells lose their myogenic capacity. The objective of my internship is to quantify the myogenic capacity of the transplanted cells by collecting samples from the 2nd transplantation (500g donors) and to analyse the results of the two transplantations. Results showed that satellite cells from 500g donors had a lower myogenic capacity than the 100g donors one. GFP fibers were mainly small fibers, regardless of the donor's weight. Therefore, these fibers have mostly hyperplastic capacities. In conclusion, our results indicate that the transplantation was effective and they confirm that the myogenic capacity of satellite cells as well as hyperplastic capacities decreases with age.
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https://hal.inrae.fr/hal-03153492
Contributor : Maryse Corvaisier <>
Submitted on : Friday, February 26, 2021 - 1:57:33 PM
Last modification on : Saturday, February 27, 2021 - 3:29:54 AM

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  • HAL Id : hal-03153492, version 1

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Morgane Chesnais. Analyse in vivo des capacités myogéniques des cellules souches musculaires par transplantation chez la truite arc-en-ciel (Oncorhynchus mykiss). [Stage] Université de Laval; IUT de Laval. 2019, 26 p. ⟨hal-03153492⟩

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