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Analysis of pig transcriptomes suggests a global regulation mechanism enabling temporary bursts of circular RNAs

Abstract : To investigate the dynamics of circRNA expression in pig testes, we designed specific strategies to individually study circRNA production from intron lariats and circRNAs originating from back-splicing of two exons. By applying these methods on seven Total-RNA-seq datasets sampled during the testicular puberty, we detected 126 introns in 114 genes able to produce circRNAs and 5,236 exonic circRNAs produced by 2,516 genes. Comparing RNA-seq our datasets to datasets from litterature (embryonic cortex and postnatal muscle stages) revealed highly abundant intronic and exonic circRNAs in one sample each in pubertal testis and embryonic cortex, respectively. This abundance was due to higher production of circRNA by the same genes in comparison to in other testis samples, rather than to the recruitment of new genes. No global relationship between circRNA and mRNA production was found. We propose ExoCirc-9244 (SMARCA5) as a marker of a particular stage in testis, which is characterized by a very low plasma estradiol level and high abundance of circRNA in testis. We hypothesize that the abundance of testicular circRNA is associated with an abrupt switch of the cellular process to overcome a particular challenge that may have arisen in the early stages of steroid production. We also hypothesize that, in certain circumstances, isoforms and circular transcripts from different genes share functions and that a global regulation of circARN production is established. Our data indicate that this massive production of circRNAs is much more related to the structure of the genes generating circRNAs than to their function.
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https://hal.inrae.fr/hal-03160320
Contributor : Sarah Maman <>
Submitted on : Friday, March 5, 2021 - 10:12:55 AM
Last modification on : Friday, June 4, 2021 - 2:40:12 PM

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Annie Robic, Sarah Djebali, Thomas Faraut, Sarah Djebali-Quelen, Rosemarie Weikard, et al.. Analysis of pig transcriptomes suggests a global regulation mechanism enabling temporary bursts of circular RNAs. RNA Biology, Taylor & Francis, 2019, 16 (9), pp.1190-1204. ⟨10.1080/15476286.2019.1621621⟩. ⟨hal-03160320⟩

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