Skip to Main content Skip to Navigation
Journal articles

Molecular mechanism for the recognition of sequence-divergent CIF peptides by the plant receptor kinases GSO1/SGN3 and GSO2

Abstract : Plants use leucine-rich repeat receptor kinases (LRR-RKs) to sense sequence diverse peptide hormones at the cell surface. A 3.0-angstrom crystal structure of the LRR-RK GSO1/SGN3 regulating Casparian strip formation in the endodermis reveals a large spiral-shaped ectodomain. The domain provides a binding platform for 21 amino acid CIF peptide ligands, which are tyrosine sulfated by the tyrosylprotein sulfotransferase TPST/SGN2. GSO1/SGN3 harbors a binding pocket for sulfotyrosine and makes extended backbone interactions with CIF2. Quantitative biochemical comparisons reveal that GSO1/SGN3-CIF2 represents one of the strongest receptor-ligand pairs known in plants. Multiple missense mutations are required to block CIF2 binding in vitro and GSO1/SGN3 function in vivo. Using structure-guided sequence analysis we uncover previously uncharacterized CIF peptides conserved among higher plants. Quantitative binding assays with known and novel CIF5 suggest that the homologous LRR-RKs GSO1/SGN3 and GSO2 have evolved unique peptide binding properties to control different developmental processes. A quantitative biochemical interaction screen, a CIF peptide antagonist and genetic analyses together implicate SERK proteins as essential coreceptor kinases required for GSO1/SGN3 and GSO2 receptor activation. Our work provides a mechanistic framework for the recognition of sequence-divergent peptide hormones in plants.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-03160352
Contributor : Christopher Lallemant <>
Submitted on : Friday, March 5, 2021 - 10:24:01 AM
Last modification on : Friday, March 5, 2021 - 10:24:03 AM

Links full text

Identifiers

Collections

Citation

Satohiro Okuda, Satoshi Fujita, Andrea Moretti, Ulrich Hohmann, Verónica Doblas, et al.. Molecular mechanism for the recognition of sequence-divergent CIF peptides by the plant receptor kinases GSO1/SGN3 and GSO2. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2020, 117 (5), pp.2693-2703. ⟨10.1073/pnas.1911553117⟩. ⟨hal-03160352⟩

Share

Metrics

Record views

7