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The mycoplasma surface proteins MIB and MIP promote the dissociation of the antibody-antigen interaction

Abstract : Mycoplasma immunoglobulin binding (MIB) and mycoplasma immunoglobulin protease (MIP) are surface proteins found in the majority of mycoplasma species, acting sequentially to capture antibodies and cleave off their V H domains. Cryo-electron microscopy structures show how MIB and MIP bind to a Fab fragment in a "hug of death" mechanism. As a result, the orientation of the V L and V H domains is twisted out of alignment, disrupting the antigen binding site. We also show that MIB-MIP has the ability to promote the dissociation of the antibody-antigen complex. This system is functional in cells and protects mycoplasmas from antibody-mediated agglutination. These results highlight the key role of the MIB-MIP system in immunity evasion by mycoplasmas through an unprecedented mechanism, and open exciting perspectives to use these proteins as potential tools in the antibody field.
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https://hal.inrae.fr/hal-03162754
Contributor : Muriel Gauthier <>
Submitted on : Monday, March 8, 2021 - 4:46:26 PM
Last modification on : Tuesday, May 11, 2021 - 4:51:34 PM

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Pierre Nottelet, Laure Bataille, Geraldine Gourgues, Robin Anger, Carole Lartigue, et al.. The mycoplasma surface proteins MIB and MIP promote the dissociation of the antibody-antigen interaction. Science Advances , American Association for the Advancement of Science (AAAS), 2021, 7 (10), pp.eabf2403. ⟨10.1126/sciadv.abf2403⟩. ⟨hal-03162754⟩

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