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A Defined Epitope on the Human Choriogonadotropin α-Subunit Interacts with the Second Extracellular Loop of the Transmembrane Domain of the Lutropidchoriogonadotropin Receptor

Abstract : The monoclonal antibody, HT13 recognizes human choriogonadotropin (CG) bound to the extracellular domain of its receptor, but not to the full-length receptor. The HT13 epitope is located in the regions of residues 15-17 and 73-75 of the human CG alpha-subunit. Only one synthetic peptide, lutropin (LH)/CG-receptor-(481-497)-peptide (EL2 peptide), which spans the second putative extracellular loop of the LH/CG-receptor endodomain, prevents recognition of human CG by HT13 mAb. EL2 peptide decreases hormone-induced cAMP production, but not high-affinity binding. An anti-EL2 serum also displays the capacity to inhibit human CG-stimulated cAMP production. These results suggest that the second extracellular loop of the receptor is in contact with the HT13 epitope of human CG alpha-subunit and is involved in signal transduction. A relative orientation of the hormone versus the endodomain is proposed.
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https://hal.inrae.fr/hal-03170897
Contributor : Eric Marchoux <>
Submitted on : Tuesday, March 16, 2021 - 3:12:29 PM
Last modification on : Tuesday, September 7, 2021 - 3:34:34 PM

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Laurence Couture, Jean-Jacques Remy, Hanitra Rabesona, Frederic Troalen, Edith Pajot-Augy, et al.. A Defined Epitope on the Human Choriogonadotropin α-Subunit Interacts with the Second Extracellular Loop of the Transmembrane Domain of the Lutropidchoriogonadotropin Receptor. European Journal of Biochemistry, 1996, 241 (2), pp.627-632. ⟨10.1111/j.1432-1033.1996.00627.x⟩. ⟨hal-03170897⟩

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