mmannot: How to improve small–RNA annotation? - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2020

mmannot: How to improve small–RNA annotation?

Résumé

High -throughput sequencing makes it possible to provide the genome-wide distribution of small non coding RNAs in a single experiment, and contributed greatly to the identification and understanding of these RNAs in the last decade. Small non coding RNAs gather a wide collection of classes, such as microRNAs, tRNA-derived fragments, small nucleolar RNAs and small nuclear RNAs, to name a few. As usual in RNA-seq studies, the sequencing step is followed by a feature quantification step: when a genome is available, the reads are aligned to the genome, their genomic positions are compared to the already available annotations, and the corresponding features are quantified. However, problem arises when many reads map at several positions and while different strategies exist to circumvent this problem, all of them are biased. In this article, we present a new strategy that compares all the reads that map at several positions, and their annotations when available. In many cases, all the hits co-localize with the same feature annotation (a duplicated miRNA or a duplicated gene, for instance). When different annotations exist for a given read, we propose to merge existing features and provide the counts for the merged features. This new strategy has been implemented in a tool, mmannot, freely available at https://github.com/ mzytnicki/mmannot.
Fichier principal
Vignette du fichier
2020_Zytnicki_plos one.pdf (2.86 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03182914 , version 1 (26-03-2021)

Licence

Paternité

Identifiants

Citer

Matthias Zytnicki, Christine Gaspin. mmannot: How to improve small–RNA annotation?. PLoS ONE, 2020, 15 (5), ⟨10.1371/journal.pone.0231738⟩. ⟨hal-03182914⟩
57 Consultations
23 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More