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Endogenous nitrogen contribution to in vitrodigestibility of animal and plant proteins

Abstract : Protein digestibility, a key indicator of dietary protein quality, can be estimated in vitro. The objectivewasto determinethe contribution of endogenous N, released by the enzyme autolysis, to in vitro protein digestibilityusing 15N-labelled proteins. Methods:15N-labelled gluten and caseins (4, 8 and 16% of the meal) were digested using the INFOGEST static in vitromodel.The endogenous N was measured after centrifugation and 10-kDa ultrafiltration, allowing the true N digestibilitydetermination. The proteolysis degree, based on the primary amine content, was determined. Results: Alarge proportion (93±11%) of the endogenous N < 10 kDa within digesta was present before digestion, with somecontribution, although limited(20-30%),of the enzyme autolysis. For both proteins, true N digestibility was not significantly different between 30and120 min of intestinal digestion,but significantly higher for the lowest protein content (4%). The proteolysis degree was more correlated to theapparentdigestibility, i.e.withno endogenous N correction, than to the true digestibility, showing that further endogenous correction is needed for more accuracy.Conclusion: In vitro protein digestibility, as well as proteolysis degree, needs to be corrected for the endogenous N contribution, although to a different extent.
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Contributor : Anne Giboulot <>
Submitted on : Friday, May 7, 2021 - 2:41:53 PM
Last modification on : Thursday, June 17, 2021 - 12:00:01 PM


Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 International License


  • HAL Id : hal-03220807, version 1


Olivia Ménard, Lucile Chauvet, Gwénaële Henry, Didier Dupont, Juliane Calvez, et al.. Endogenous nitrogen contribution to in vitrodigestibility of animal and plant proteins. Virtual International Conference on Food Digestion ICFD 2021, Cost Infogest, May 2021, the Virtual International Conference on Food Digestion (#VICFD2021) on 6-7th May 2021., France. ⟨hal-03220807⟩



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