Hormonal control of glucose production and pyruvate kinase activity in isolated rat liver cells: influence of hypothyroidism - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Molecular and Cellular Endocrinology Année : 1987

Hormonal control of glucose production and pyruvate kinase activity in isolated rat liver cells: influence of hypothyroidism

Résumé

Hormonal control of glucose production and of L-pyruvate kinase activity has been measured in isolated liver cells from fed control and thyroidectomized rats. In hypothyroid rats, sensitivity to isoproterenol as measured by these parameters was increased: the apparent K0.5 for isoproterenol-induced stimulation of glucose production decreased from 8.0 +/- 3 X 10(-6) M in control rats to 2.0 +/- 0.2 X 10(-8) M in hypothyroid rats (P less than 0.001) and the apparent K0.5 for inhibition of L-pyruvate kinase was 5 +/- 2 X 10(-7) M vs. 7 +/- 2 X 10(-9) M (P less than 0.001) in control and thyroidectomized rats, respectively. Utilisation of specific adrenergic antagonists confirmed increased beta-adrenergic responsiveness in hypothyroid rats. This phenomenon was not reversed by 3 days of T3 treatment (10 micrograms/100 g body weight). Sensitivity to the alpha-agonist was unchanged by thyroid status. Stimulation of glucose production and inhibition of L-pyruvate kinase activity by glucagon and their reversal by insulin were not affected by hypothyroidism. The dose-response curve to vasopressin and its maximal effect measured on stimulation of glucose production were unchanged in thyroidectomized rats. Thus, hypothyroidism produces a specific enhancement of liver beta-adrenergic responsiveness without affecting sensitivity to glucagon, insulin and vasopressin.

Dates et versions

hal-03275004 , version 1 (30-06-2021)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

Citer

M. Laville, Y. Khalfallah, H. Vidai, M. Beylot, Blandine Comte, et al.. Hormonal control of glucose production and pyruvate kinase activity in isolated rat liver cells: influence of hypothyroidism. Molecular and Cellular Endocrinology, 1987, 50 (3), pp.247-253. ⟨10.1016/0303-7207(87)90023-2⟩. ⟨hal-03275004⟩

Collections

INRAE
8 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More