Skip to Main content Skip to Navigation
Journal articles

Distinct barcodes for the Cereal leaf beetles Oulema melanopus and Oulema duftschmidi (Coleoptera: Chrysomelidae), two syntopical sibling species

Abstract : Oulema melanopus (Linnaeus, 1758) and Oulema duftschmidi (Redtenbacher, 1874) (Coleoptera: Chrysomelidae) are two native West Palaearctic species developing on various cultivated and wild grasses. Along with O. obscura they are considered to be secondary pests of cereal crops. However, local outbreaks have been recorded recently and their status as secondary pests may evolve, especially as the use of broad-spectrum insecticides is now greatly reduced. Oulema melanopus and 0. duftschmidi are considered to be sibling species. They are morphologically very close and difficult to distinguish from each other, which makes it difficult to study them. We tested the reliability of the standard barcode fragment (COI) for distinguishing between these species. A total of 92 samples of the two species, covering the majority of their natural range, was sequenced for the barcode fragment and inter- and intraspecific genetic distances were estimated. Our results confirm those of Bezdek & Baselga (2015, Acta Entomol. Mus. Nat. Prag. 55: 273-304) in that this marker cannot differentiate between all the species of the Oulema melanopus complex, which in the Mediterranean basin contains several described and possibly some undescribed cryptic species. However, this marker may be useful in an agricultural context in areas where only O. melanopus and O. duftschmidi occur (such as in cereal crops in France) where it can be used to reliably and rapidly separate all stages of these two taxa and can therefore help in studying their ecology and dynamics.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-03276037
Contributor : Christine Riou Connect in order to contact the contributor
Submitted on : Thursday, July 1, 2021 - 3:56:08 PM
Last modification on : Friday, September 24, 2021 - 3:58:00 PM

File

EJE_eje-202001-0052.pdf
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Julie Leroy, Jean-David Chapelin-Viscardi, Guenaelle Genson, Julien Haran, Eric Pierre, et al.. Distinct barcodes for the Cereal leaf beetles Oulema melanopus and Oulema duftschmidi (Coleoptera: Chrysomelidae), two syntopical sibling species. European Journal of Entomology, Czech Entomological Society, 2020, 117, pp.490-503. ⟨10.14411/eje.2020.052⟩. ⟨hal-03276037⟩

Share

Metrics

Record views

66

Files downloads

100