The current study extends the faunistic survey ofTrichogrammaspecies parasitizing ECB to all agricultural growing regions in Serbia. Specimens ofTrichogrammawere reared from parasitized egg masses of ECB collected from field-grown corn and pepper crops. The number of egg masses parasitized varied by location. Using sequences of mitochondrial cytochrome oxidase I (COI) gene, we examined intra and interspecific variation inTrichogrammaparasitizing the ECB egg masses. Seventy specimens were successfully sequenced of which, 65 were identified asT. brassicae. The remaining five were identified asT. evanescens, and this second species was only collected from pepper.