Skip to Main content Skip to Navigation
Journal articles

Transcription from a gene desert in a melanoma porcine model

Abstract : The genetic mechanisms underlying cutaneous melanoma onset and progression need to be further understood to improve patients' care. Several studies have focused on the genetic determinism of melanoma development in the MeLiM pig, a biomedical model of cutaneous melanoma. The objective of this study was to better describe the influence of a particular genomic region on melanoma progression in the MeliM model. Indeed, a large region of theSus scrofachromosome 1 has been identified by linkage and association analyses, but the causal mechanisms have remained elusive. To deepen the analysis of this candidate region, a dedicated SNP panel was used to fine map the locus, downsizing the interval to less than 2 Mb, in a genomic region located within a large gene desert. Transcription from this locus was addressed using a tiling array strategy and further validated by RT-PCR in a large panel of tissues. Overall, the gene desert showed an extensive transcriptional landscape, notably dominated by repeated element transcription in tumor and fetal tissues. The transcription of LINE-1 and PERVs has been confirmed in skin and tumor samples from MeLiM pigs. In conclusion, although this study still does not identify a candidate mutation for melanoma occurrence or progression, it highlights a potential role of repeated element transcriptional activity in the MeLiM model.
Complete list of metadata

https://hal.inrae.fr/hal-03285890
Contributor : Roselyne Tâche Connect in order to contact the contributor
Submitted on : Tuesday, July 13, 2021 - 4:43:29 PM
Last modification on : Thursday, July 15, 2021 - 3:33:39 AM

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

S. Marthey, J. Estellé, A. Blin, P. Wahlberg, F. Créchet, et al.. Transcription from a gene desert in a melanoma porcine model. Molecular Genetics and Genomics, Springer Verlag, 2020, 295 (5), pp.1239-1252. ⟨10.1007/s00438-020-01694-6⟩. ⟨hal-03285890⟩

Share

Metrics

Record views

44