Skip to Main content Skip to Navigation
Journal articles

Interferon-Induced Protein 44 and Interferon-Induced Protein 44-Like Restrict Replication of Respiratory Syncytial Virus

Abstract : Cellular intrinsic immunity, mediated by the expression of an array of interferon-stimulated antiviral genes, is a vital part of host defense. We have previously used a bioinformatic screen to identify two interferon-stimulated genes (ISG) with poorly characterized function, interferon-induced protein 44 (IFI44) and interferoninduced protein 44-like (IFI44L), as potentially being important in respiratory syncytial virus (RSV) infection. Using overexpression systems, CRISPR-Cas9-mediated knockout, and a knockout mouse model, we investigated the antiviral capability of these genes in the control of RSV replication. Overexpression of IFI44 or IFI44L was sufficient to restrict RSV infection at an early time postinfection. Knocking out these genes in mammalian airway epithelial cells increased levels of infection. Both genes express antiproliferative factors that have no effect on RSV attachment but reduce RSV replication in a minigenome assay. The loss of Ifi44 was associated with a more severe infection phenotype in a mouse model of infection. These studies demonstrate a function for IFI44 and IFI44L in controlling RSV infection.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-03318538
Contributor : Camille Serva <>
Submitted on : Tuesday, August 10, 2021 - 11:45:52 AM
Last modification on : Friday, August 13, 2021 - 3:26:10 AM

File

2020_Busse_JVI.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

D. Busse, D. Habgood-Coote, S. Clare, C. Brandt, I. Bassano, et al.. Interferon-Induced Protein 44 and Interferon-Induced Protein 44-Like Restrict Replication of Respiratory Syncytial Virus. Journal of Virology, American Society for Microbiology, 2020, 94 (18), ⟨10.1128/jvi.00297-20⟩. ⟨hal-03318538⟩

Share

Metrics

Record views

14

Files downloads

9