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Identification and characterization of a new Type III polyketide synthase from a marine yeast, Naganishia uzbekistanensis

Abstract : A putative Type III Polyketide synthase (PKSIII) encoding gene was identified from a marine yeast, Naganishia uzbekistanensis strain Mo29 (UBOCC-A-208024) (formerly named as Cryptococcus sp.) isolated from deep-sea hydrothermal vents. This gene is part of a distinct phylogenetic branch compared to all known terrestrial fungal sequences. This new gene encodes a C-terminus extension of 74 amino acids compared to other known PKSIII proteins like Neurospora crassa. Full-length and reduced versions of this PKSIII were successfully cloned and overexpressed in a bacterial host, Escherichia coli BL21 (DE3). Both proteins showed the same activity, suggesting that additional amino acid residues at the C-terminus are probably not required for biochemical functions. We demonstrated by LC-ESI-MS/MS that these two recombinant PKSIII proteins could only produce tri- and tetraketide pyrones and alkylresorcinols using only long fatty acid chain from C8 to C16 acyl-CoAs as starter units, in presence of malonyl-CoA. In addition, we showed that some of these molecules exhibit cytotoxic activities against several cancer cell lines.
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https://hal.inrae.fr/hal-03321249
Contributor : Marina Pavlidès <>
Submitted on : Tuesday, August 17, 2021 - 12:17:54 PM
Last modification on : Tuesday, August 24, 2021 - 10:35:05 AM

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Laure Martinelli, Vanessa Redou, Bastien Cochereau, Ludovic Delage, Nolwenn Hymery, et al.. Identification and characterization of a new Type III polyketide synthase from a marine yeast, Naganishia uzbekistanensis. Marine drugs, MDPI, 2020, 18 (12), 21 p. ⟨10.3390/md18120637⟩. ⟨hal-03321249⟩

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