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Involvement of Cis-acting elements in molecular regulation of JH-mediated vitellogenin gene 2 of female Periplaneta americana

Abstract : Vitellogenins (Vgs) are yolk protein precursors that are regulated by juvenile hormone (JH) and/or 20-hydroxyecdysone (20E) in insects. JH acts as the principal gonadotropin that stimulates vitellogenesis in hemimetabolous insects. In this study, we cloned and characterized the Periplaneta americana Vitellogenin 2 ( Vg2 ) promoter. Multiple sites for putative transcription factor binding were predicted for the 1,804 bp Vg2 promoter region, such as the Broad-Complex, ecdysone response element (EcRE), GATA, Hairy, JH response element (JHRE), and Methoprene (Met)-binding motif, among others. Luciferase reporter assay has identified that construct −177 bp is enough to support JH III induction but not 20E suppression. This 38 bp region (from −177 to −139 bp) contains two conserved response element half-sites separated by 2 nucleotides spacer (DR2) and is designated as Vg2 RE ( −168 GAGTCACGGAGTCGCCGCTG −149 ). Mutation assay and luciferase assay data using mutated constructs verified the crucial role of G residues in Vg2 RE for binding the isolated fat body nuclear protein. In Sf 9 cells, a luciferase reporter placed under the control of a minimal promoter containing Vg2 RE was induced by JH III in a dose- and time-dependent manner. Nuclear proteins isolated from previtellogenic female fat body cells bound to Vg2 RE, and this binding was outcompeted by a 50-fold excess of cold Drosophila melanogaster DR4 and Galleria mellonella JH binding protein response elements (Chorion factor-I/Ultraspiracle). Affinity pull-down experiment with nuclear extracts of previtellogenic female fat body, using 31-bp probe Vg2 RE as bait, yielded a 71 kDa candidate nuclear protein that may mediate the regulatory action of the JH III.
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https://hal.inrae.fr/hal-03335815
Contributor : Bernard Duvic <>
Submitted on : Monday, September 6, 2021 - 3:10:16 PM
Last modification on : Monday, September 20, 2021 - 5:30:01 PM

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Azza Elgendy, Amr Mohamed, Bernard Duvic, Muhammad Tufail, Makio Takeda. Involvement of Cis-acting elements in molecular regulation of JH-mediated vitellogenin gene 2 of female Periplaneta americana. Frontiers in Physiology, Frontiers, 2021, 12 (1350), pp.723072. ⟨10.3389/fphys.2021.723072⟩. ⟨hal-03335815⟩

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