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Full Length Transcriptome Highlights the Coordination of Plastid Transcript Processing

Abstract : Plastid gene expression involves many post-transcriptional maturation steps resulting in a complex transcriptome composed of multiple isoforms. Although short read RNA-seq has considerably improved our understanding of the molecular mechanisms controlling these processes, it is unable to sequence full-length transcripts. This information is however crucial when it comes to understand the interplay between the various steps of plastid gene expression. Here, the study of the Arabidopsis leaf plastid transcriptome using Nanopore sequencing showed that many splicing and editing events were not independent but co-occurring. For a given transcript, maturation events also appeared to be chronologically ordered with splicing happening after most sites are edited.
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Preprints, Working Papers, ...
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https://hal.inrae.fr/hal-03343771
Contributor : Etienne Delannoy <>
Submitted on : Tuesday, September 14, 2021 - 1:56:38 PM
Last modification on : Thursday, September 16, 2021 - 3:38:49 AM

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Marine Guilcher, Arnaud Liehrmann, Chloé Seyman, Thomas Blein, Guillem Rigaill, et al.. Full Length Transcriptome Highlights the Coordination of Plastid Transcript Processing. 2021. ⟨hal-03343771⟩

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