Characterization of glucansucrase and dextran from Weissella sp TN610 with potential as safe food additives - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue International Journal of Biological Macromolecules Année : 2013

Characterization of glucansucrase and dextran from Weissella sp TN610 with potential as safe food additives

Résumé

Pear-derived Weissella sp. TN610 produced extracellular glycosyltransferase activity responsible for the synthesis of soluble exopolysaccharide from sucrose. Acid and dextranase-catalyzed hydrolysis revealed that the synthesized polymer was a glucan. According to H-1 and C-13 NMR analysis, the glucan produced by TN610 was a linear dextran made of 96% alpha-(1 -> 6) and 4% alpha-(1 -> 3) linkages. Zymogram analysis confirmed the presence of a unique glucansucrase of approximately 180 kDa in the cell-free supernatant from TN610. The crude enzyme, optimally active at 37 degrees C and pH 5, has promising potential for application as a food additive since it catalyzes dextran synthesis in sucrose-supplemented milk, allowing its solidification. A 4257-bp product corresponding to the mature glucansucrase gene was amplified by PCR from TN610. It encoded a polypeptide of 1418 residues having a calculated molecular mass of 156.089 kDa and exhibiting 96% and 95% identity with glucansucrases from Lactobacillus fermentum Kg3 and Weissella cibaria CMU, respectively. (C) 2012 Elsevier B.V. All rights reserved.

Dates et versions

hal-01004509 , version 1 (11-06-2014)

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Wacim W. Bejar, Valerie V. Gabriel, Myriam M. Amari, Sandrine Morel, Monia M. Mezghani, et al.. Characterization of glucansucrase and dextran from Weissella sp TN610 with potential as safe food additives. International Journal of Biological Macromolecules, 2013, 52, pp.125 - 132. ⟨10.1016/j.ijbiomac.2012.09.014⟩. ⟨hal-01004509⟩
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