<em>csp</em>-like genes of <em>Lactobacillus delbrueckii</em> ssp. <em>bulgaricus</em> and their response to cold shock - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue FEMS Microbiology Letters Année : 2003

csp-like genes of Lactobacillus delbrueckii ssp. bulgaricus and their response to cold shock

Résumé

The two csp-like genes from the lactic acid bacterium Lactobacillus delbrueckii ssp. bulgaricus were characterized and designated cspA and cspB. The gene cspA has been identified using a polymerase chain reaction (PCR)-based approach with degenerated primers and further characterized using an inverse PCR strategy. cspA encodes a protein of 65 amino acid residues which displays between 81 and 77% identity with proteins CspL and CspP of Lactobacillus plantarum. cspB has been identified as a cspA ortholog using the partial sequence of the L. bulgaricus ATCC11842. cspB encodes a protein of 69 amino acids which has 42% identity with CspA. Northern blot analyses showed that cspA is transcribed as a single gene and that its transcription increased after a temperature downshift from 42 to 25 degrees C. In contrast, cspB is part of an operon transcribed at constant level irrespective of the temperature. These results indicate that cspA encodes the only Csp-like protein of L. bulgaricus induced by a downshift of temperature.

Dates et versions

hal-02679208 , version 1 (31-05-2020)

Identifiants

Citer

Pascale Serror, Rozenn Dervyn, S. Dusko Ehrlich, Emmanuelle Maguin. csp-like genes of Lactobacillus delbrueckii ssp. bulgaricus and their response to cold shock. FEMS Microbiology Letters, 2003, 226 (2), pp.323-330. ⟨10.1016/S0378-1097(03)00594-9⟩. ⟨hal-02679208⟩

Collections

INRA INRAE
22 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More