Saccharomyces Cerevisiae Var Boulardii CNCM I–1079 Reduces Expression of Genes Involved in Inflammatory Response in Porcine Cells Challenged by Enterotoxigenic E. Coli and Influences Bacterial Communities in an In Vitro Model of the Weaning Piglet Colon
Résumé
Enterotoxigenic Escherichia coli (ETEC) is the main infectious agent responsible for piglet
post-weaning diarrhea with high mortality rates. Antimicrobials represent the current principal strategy for treating ETEC infections in pig farms, but the occurrence of multi-resistant bacterial strains
has considerably increased in the last decades. Thus, finding non-antibiotic alternatives becomes a
real emergency. In this context, we investigated the effect of a live yeast strain, Saccharomyces cerevisiae
var boulardii CNCM I-1079 (SB) in an in vitro model of the weaning piglet colon implemented with
a mucus phase (MPigut-IVM) inoculated with ETEC and coupled with an intestinal porcine cell
line IPI-2I. We showed that SB was able to modulate the in vitro microbiota through an increase
in Bacteroidiaceae and a decrease in Prevotellaceae families. Effluents collected from the SB treated
bioreactors were able to mitigate the expression level of genes encoding non-gel forming mucins, tight
junction proteins, innate immune pathway, and pro-inflammatory response in IPI-2I cells. Furthermore, SB exerted a significant protective effect against ETEC adhesion on porcine IPEC-J2 intestinal
cells in a dose-dependent manner and showed a positive effect on ETEC-challenged IPEC-J2 by
lowering expression of genes involved in pro-inflammatory immune responses. Our results showed
that the strain SB CNCM I-1079 could prevent microbiota dysbiosis associated with weaning and
protect porcine enterocytes from ETEC infections by reducing bacterial adhesion and modulating the
inflammatory response
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