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Article Dans Une Revue Journal of Chromatography B Biomedical Sciences and Applications Année : 1997

Gas chromatographic–mass spectrometric identification of main metabolites of stanozolol in cattle after oral and subcutaneous administration

Résumé

An analytical method has been developed in order to control the illegal use of stanozolol as growth promoter in livestock. The procedure was based on enzymatic hydrolysis, purification on a Clean Screen DAU column and derivatization with heptafluorobutyric anhydride prior to GC–MS analysis. This method allowed us to study the metabolism of stanozolol in cattle after oral and subcutaneous administrations. Urinary metabolites were identified by mass spectrometry. Stanozolol and 16-hydroxystanozolol were detected after oral administration, while 16-hydroxystanozolol and 4,16-dihydroxystanozolol were found after subcutaneous administration.

Dates et versions

hal-03550488 , version 1 (01-02-2022)

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Veronique Ferchaud-Roucher, Bruno Le Bizec, Marie-Pierre Montrade, Daniel Maume, Fabrice Monteau, et al.. Gas chromatographic–mass spectrometric identification of main metabolites of stanozolol in cattle after oral and subcutaneous administration. Journal of Chromatography B Biomedical Sciences and Applications, 1997, 695 (2), pp.269-277. ⟨10.1016/s0378-4347(97)00124-2⟩. ⟨hal-03550488⟩
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