Studies on the determination of chlorotestosterone and its metabolites in bovine urine† - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Analyst Year : 1998

Studies on the determination of chlorotestosterone and its metabolites in bovine urine†

Abstract

Chlorotestosterone and its metabolites were determined in urine samples from bovine animals treated with chlorotestosterone acetate by oral and intramuscular routes. Sample preparation, involving enzymatic deconjugation and solid-phase extraction, was optimised. The effect of different enzyme preparations, pH, and time of incubation were studied. An extraction/clean-up procedure based on solid-phase extraction (C18 cartridge) and liquid–liquid clean-up was developed. Determination of chlorotestosterone and its metabolites was by enzyme immunoassay and GC-MS. Metabolites were converted into their TMS-enol-TMS-ether and TMS-oxime-TMS-ether forms before GC-MS (EI) analysis.

Dates and versions

hal-03550674 , version 1 (01-02-2022)

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Michaela Walshe, Michael O’keeffe, Bruno Le Bizec. Studies on the determination of chlorotestosterone and its metabolites in bovine urine†. Analyst, 1998, 123 (12), pp.2687-2691. ⟨10.1039/A805125I⟩. ⟨hal-03550674⟩
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