Drosophila suzukii is an invasive pest which became a serious threat to stone and berry fruit production in Europe. Knowledge about the host range of this pest and the effect of the succession of available hosts over time is however lacking in vineyard landscapes. Our study aimed to evaluate the host range of D. suzukii throughout the year in a vineyard-dominated area. We assessed egg deposition and emergence on nine wild and cultivated plant species sampled in twenty vineyard landscapes during two years. The temporal dynamics of D. suzukii presence was observed on five fruit species (Viscum album L., Prunus avium L., Sambucus nigra L., Rubus fruticosus L. aggr. and Vitis vinifera L. cv. Merlot) with different phenologies. Infestations on host plants depended on the season and the proportion of other fruits species in the landscape around the vineyard plots. The data collected enabled us to provide estimates of the population dynamics among different hosts and generations of the pest and to propose a pattern of temporal succession of host plant species adapted to our regional and agroecological context.