Optimizing Rhizobium- legume symbioses by simultaneous measurement of rhizobial competitiveness and N 2 fixation in nodules - Archive ouverte HAL Access content directly
Journal Articles Proceedings of the National Academy of Sciences of the United States of America Year : 2020

Optimizing Rhizobium- legume symbioses by simultaneous measurement of rhizobial competitiveness and N 2 fixation in nodules

, , (1) , (2) , (3) , (1) , (1)
1
2
3

Abstract

Legumes tend to be nodulated by competitive rhizobia that do not maximize nitrogen (N 2 ) fixation, resulting in suboptimal yields. Rhizobial nodulation competitiveness and effectiveness at N 2 fixation are independent traits, making their measurement extremely time-consuming with low experimental throughput. To transform the experimental assessment of rhizobial competitiveness and effectiveness, we have used synthetic biology to develop reporter plasmids that allow simultaneous high-throughput measurement of N 2 fixation in individual nodules using green fluorescent protein (GFP) and barcode strain identification (Plasmid ID) through next generation sequencing (NGS). In a proof-of-concept experiment using this technology in an agricultural soil, we simultaneously monitored 84 different Rhizobium leguminosarum strains, identifying a supercompetitive and highly effective rhizobial symbiont for peas. We also observed a remarkable frequency of nodule coinfection by rhizobia, with mixed occupancy identified in ∼20% of nodules, containing up to six different strains. Critically, this process can be adapted to multiple Rhizobium -legume symbioses, soil types, and environmental conditions to permit easy identification of optimal rhizobial inoculants for field testing to maximize agricultural yield.

Dates and versions

hal-03695913 , version 1 (15-06-2022)

Identifiers

Cite

Marcela Mendoza-Suárez, Barney Geddes, Carmen Sánchez-Cañizares, Ricardo Ramírez-González, Charlotte Kirchhelle, et al.. Optimizing Rhizobium- legume symbioses by simultaneous measurement of rhizobial competitiveness and N 2 fixation in nodules. Proceedings of the National Academy of Sciences of the United States of America, 2020, 117 (18), pp.9822-9831. ⟨10.1073/pnas.1921225117⟩. ⟨hal-03695913⟩

Collections

INRAE
3 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More